We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1y99

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1y99.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1y99.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1y99| PDB=1y99 | SCENE= }}
{{STRUCTURE_1y99| PDB=1y99 | SCENE= }}
-
'''HIV-1 subtype A DIS RNA duplex'''
+
===HIV-1 subtype A DIS RNA duplex===
-
==Overview==
+
<!--
-
BACKGROUND: An important step in retroviral replication is dimerization of the genomic RNA prior to encapsidation. Dimerization is initiated by the formation of a transient 'kissing-loop complex' that is thought to be subsequently matured into an extended duplex by the nucleocapsid protein (NCp). Although chemical probing and nuclear magnetic resonance spectroscopy have provided insight into the structure of the kissing-loop structure, no structural information concerning the extended-duplex state is available so far. RESULTS: The structure of a minimal HIV-1 RNA dimerization initiation site has been solved at 2.3 A resolution in two different space groups. It reveals a 22 base pair extended duplex with two noncanonical Watson-Crick-like G-A mismatches, each adjacent to a bulged-out adenine. The structure shows significant asymmetry in deep groove width and G-A base-pair conformations. A network of eight magnesium cations was clearly identified, one being unusually chelated by the 3' phosphate of each bulge across an extremely narrowed deep major groove. CONCLUSIONS: These crystal structures represent the putative matured form of the initial kissing-loop complex. They show the ability of this self-complementary RNA hairpin loop to acquire a more stable extended duplex structure. Both bulged adenines form a striking 'base grip' that could be a recognition signal, either in cis for another viral RNA sequence, or in trans for a protein, possibly the NCp. Magnesium binding might be important to promote and stabilize the observed extrahelical conformation of these bulges.
+
The line below this paragraph, {{ABSTRACT_PUBMED_10574792}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 10574792 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_10574792}}
==About this Structure==
==About this Structure==
Line 31: Line 35:
[[Category: Magnesium]]
[[Category: Magnesium]]
[[Category: Rna]]
[[Category: Rna]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:02:04 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:07:29 2008''

Revision as of 14:07, 28 July 2008

Image:1y99.png

Template:STRUCTURE 1y99

HIV-1 subtype A DIS RNA duplex

Template:ABSTRACT PUBMED 10574792

About this Structure

Full crystallographic information is available from OCA.

Reference

The crystal structure of the dimerization initiation site of genomic HIV-1 RNA reveals an extended duplex with two adenine bulges., Ennifar E, Yusupov M, Walter P, Marquet R, Ehresmann B, Ehresmann C, Dumas P, Structure. 1999 Nov 15;7(11):1439-49. PMID:10574792

Page seeded by OCA on Mon Jul 28 17:07:29 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1y99"
Personal tools