1ybk

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{{STRUCTURE_1ybk| PDB=1ybk | SCENE= }}
{{STRUCTURE_1ybk| PDB=1ybk | SCENE= }}
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'''RHCC cocrystallized with CAPB'''
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===RHCC cocrystallized with CAPB===
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==Overview==
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Crystals of excellent quality are a prerequisite for high-resolution X-ray data. However, in refinement protocols of crystallization conditions it is often difficult to obtain the right combination of, for example, protein concentration, drop size, temperature and additives. A novel approach for optimizing crystal contacts in a most favourable fashion by performing crystallization setups with the zwitterionic surfactant cocoamidoproylbetaine (CAPB) is introduced. In the presence of this surfactant, highly diffracting crystals were obtained. Here, data from a right-handed coiled coil (RHCC) in complex with CAPB at 1.4 A resolution are presented. The addition of CAPB using otherwise identical crystallization conditions and the same X-ray source caused an improvement in resolution from 2.9 to 1.4 A.
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{{ABSTRACT_PUBMED_15805603}}
==About this Structure==
==About this Structure==
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[[Category: Stetefeld, J.]]
[[Category: Stetefeld, J.]]
[[Category: Right hand coiled coil]]
[[Category: Right hand coiled coil]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:00:05 2008''

Revision as of 08:00, 28 July 2008

Image:1ybk.png

Template:STRUCTURE 1ybk

RHCC cocrystallized with CAPB

Template:ABSTRACT PUBMED 15805603

About this Structure

1YBK is a Single protein structure of sequence from Staphylothermus marinus. Full crystallographic information is available from OCA.

Reference

Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB)., Ozbek S, Muller JF, Figgemeier E, Stetefeld J, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):477-80. Epub 2005, Mar 24. PMID:15805603

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