1ye4

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1ye4.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1ye4.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1ye4| PDB=1ye4 | SCENE= }}
{{STRUCTURE_1ye4| PDB=1ye4 | SCENE= }}
-
'''Crystal structure of the Lys-274 to Arg mutant of Candida tenuis xylose reductase (AKR2B5) bound to NAD+'''
+
===Crystal structure of the Lys-274 to Arg mutant of Candida tenuis xylose reductase (AKR2B5) bound to NAD+===
-
==Overview==
+
<!--
-
Aldo-keto reductases of family 2 employ single site replacement Lys--&gt;Arg to switch their cosubstrate preference from NADPH to NADH. X-ray crystal structures of Lys-274--&gt;Arg mutant of Candida tenuis xylose reductase (AKR2B5) bound to NAD+ and NADP+ were determined at a resolution of 2.4 and 2.3A, respectively. Due to steric conflicts in the NADP+-bound form, the arginine side chain must rotate away from the position of the original lysine side chain, thereby disrupting a network of direct and water-mediated interactions between Glu-227, Lys-274 and the cofactor 2'-phosphate and 3'-hydroxy groups. Because anchoring contacts of its Glu-227 are lost, the coenzyme-enfolding loop that becomes ordered upon binding of NAD(P)+ in the wild-type remains partly disordered in the NADP+-bound mutant. The results delineate a catalytic reaction profile for the mutant in comparison to wild-type.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15670843}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15670843 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15670843}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Wilson, D K.]]
[[Category: Wilson, D K.]]
[[Category: Beta-alpha-barrel akr aldo-keto reductase coenzyme specificity nad]]
[[Category: Beta-alpha-barrel akr aldo-keto reductase coenzyme specificity nad]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:12:43 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:40:56 2008''

Revision as of 08:40, 29 July 2008

Image:1ye4.png

Template:STRUCTURE 1ye4

Crystal structure of the Lys-274 to Arg mutant of Candida tenuis xylose reductase (AKR2B5) bound to NAD+

Template:ABSTRACT PUBMED 15670843

About this Structure

1YE4 is a Single protein structure of sequence from Candida tenuis. Full crystallographic information is available from OCA.

Reference

Fine tuning of coenzyme specificity in family 2 aldo-keto reductases revealed by crystal structures of the Lys-274-->Arg mutant of Candida tenuis xylose reductase (AKR2B5) bound to NAD+ and NADP+., Leitgeb S, Petschacher B, Wilson DK, Nidetzky B, FEBS Lett. 2005 Jan 31;579(3):763-7. PMID:15670843

Page seeded by OCA on Tue Jul 29 11:40:56 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ye4"
Personal tools