1ygm

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[[Image:1ygm.gif|left|200px]]
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{{STRUCTURE_1ygm| PDB=1ygm | SCENE= }}
{{STRUCTURE_1ygm| PDB=1ygm | SCENE= }}
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'''NMR structure of Mistic'''
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===NMR structure of Mistic===
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==Overview==
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Although structure determination of soluble proteins has become routine, our understanding of membrane proteins has been limited by experimental bottlenecks in obtaining both sufficient yields of protein and ordered crystals. Mistic is an unusual Bacillus subtilis integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery. Using paramagnetic probes, we determined by nuclear magnetic resonance (NMR) spectroscopy that the protein forms a helical bundle with a surprisingly polar lipid-facing surface. Additional experiments suggest that Mistic can be used for high-level production of other membrane proteins in their native conformations, including many eukaryotic proteins that have previously been intractable to bacterial expression.
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(as it appears on PubMed at http://www.pubmed.gov), where 15731457 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15731457}}
==About this Structure==
==About this Structure==
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1YGM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA].
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1YGM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA].
==Reference==
==Reference==
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[[Category: Alpha-helical bundle]]
[[Category: Alpha-helical bundle]]
[[Category: Integral membrane protein]]
[[Category: Integral membrane protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:17:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:44:14 2008''

Revision as of 14:44, 27 July 2008

Image:1ygm.png

Template:STRUCTURE 1ygm

NMR structure of Mistic

Template:ABSTRACT PUBMED 15731457

About this Structure

1YGM is a Single protein structure of sequence from Bacillus subtilis subsp. subtilis str. 168. Full experimental information is available from OCA.

Reference

NMR structure of Mistic, a membrane-integrating protein for membrane protein expression., Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S, Science. 2005 Feb 25;307(5713):1317-21. PMID:15731457

Page seeded by OCA on Sun Jul 27 17:44:14 2008

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