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| - | [[Image:1ygt.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1ygt| PDB=1ygt | SCENE= }} | | {{STRUCTURE_1ygt| PDB=1ygt | SCENE= }} |
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| - | '''Dynein Light Chain TcTex-1'''
| + | ===Dynein Light Chain TcTex-1=== |
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| - | ==Overview==
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| - | TcTex-1, one of three dynein light chains of the dynein motor complex, has been implicated in targeting and binding cargoes to cytoplasmic dynein for retrograde or apical transport. Interactions between TcTex-1 and a diverse set of proteins such as the dynein intermediate chain, Fyn, DOC2, FIP1, the poliovirus receptor, CD155, and the rhodopsin cytoplasmic tail have been reported; yet, despite the broad range of targets, a consensus binding sequence remains uncertain. Consequently, we have solved the crystal structure of the full-length Drosophila homolog of TcTex-1 to 1.7 A resolution using MAD phasing to gain insight into its function and target specificity. The structure is homodimeric with a domain swapping of beta-strand 2 and has a fold similar to the dynein light chain, LC8. Based on structural alignment, the TcTex-1 and LC8 sequences show no identity, although the root mean square deviation between secondary structural elements is less than 1.6 A. Moreover, the N terminus, which is equivalent to beta-strand 1 in LC8, is splayed out and binds to a crystallographic dimer as an anti-parallel beta-strand at the same position as the neuronal nitric-oxide synthase peptide in the LC8 complex. Similarity to LC8 and comparison to the LC8-neuronal nitricoxide synthase complex suggest that TcTex-1 binds its targets in a similar manner as LC8 and provides insight to the lack of strict sequence identity among the targets for TcTex-1.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_15701632}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15701632 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15701632}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Xie, H.]] | | [[Category: Xie, H.]] |
| | [[Category: Domain swapping]] | | [[Category: Domain swapping]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:17:59 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:12:29 2008'' |
Revision as of 19:12, 27 July 2008
Template:STRUCTURE 1ygt
Dynein Light Chain TcTex-1
Template:ABSTRACT PUBMED 15701632
About this Structure
1YGT is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Crystal structure of dynein light chain TcTex-1., Williams JC, Xie H, Hendrickson WA, J Biol Chem. 2005 Jun 10;280(23):21981-6. Epub 2005 Feb 8. PMID:15701632
Page seeded by OCA on Sun Jul 27 22:12:29 2008
