1yht

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[[Image:1yht.gif|left|200px]]
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{{STRUCTURE_1yht| PDB=1yht | SCENE= }}
{{STRUCTURE_1yht| PDB=1yht | SCENE= }}
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'''Crystal structure analysis of Dispersin B'''
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===Crystal structure analysis of Dispersin B===
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==Overview==
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Bacteria in a biofilm are enmeshed in a self-synthesized extracellular polysaccharide matrix that holds the bacteria together in a mass and firmly attaches the bacterial mass to the underlying surface. A major component of the extracellular polysaccharide matrix in several phylogenetically diverse bacteria is PGA, a linear polymer of N-acetylglucosamine residues in beta(1,6)-linkage. PGA is produced by the Gram-negative periodontopathogen Actinobacillus actinomycetemcomitans as well as by the Gram-positive device-associated pathogen Staphylococcus epidermidis. We recently reported that A.actinomycetemcomitans produces a soluble glycoside hydrolase named dispersin B, which degrades PGA. Here, we present the crystal structure of dispersin B at 2.0A in complex with a glycerol and an acetate ion at the active site. The enzyme crystallizes in the orthorhombic space group C222(1) with cell dimensions a=41.02A, b=86.13A, c=185.77A. The core of the enzyme consists a (beta/alpha)(8) barrel topology similar to other beta-hexosaminidases but significant differences exist in the arrangement of loops hovering in the vicinity of the active site. The location and interactions of the glycerol and acetate moieties in conjunction with the sequence analysis suggest that dispersin B cleaves beta(1,6)-linked N-acetylglucosamine polymer using a catalytic machinery similar to other family 20 hexosaminidases which cleave beta(1,4)-linked N-acetylglucosamine residues.
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The line below this paragraph, {{ABSTRACT_PUBMED_15878175}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15878175 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15878175}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structural analysis of dispersin B, a biofilm-releasing glycoside hydrolase from the periodontopathogen Actinobacillus actinomycetemcomitans., Ramasubbu N, Thomas LM, Ragunath C, Kaplan JB, J Mol Biol. 2005 Jun 10;349(3):475-86. Epub 2005 Apr 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15878175 15878175]
Structural analysis of dispersin B, a biofilm-releasing glycoside hydrolase from the periodontopathogen Actinobacillus actinomycetemcomitans., Ramasubbu N, Thomas LM, Ragunath C, Kaplan JB, J Mol Biol. 2005 Jun 10;349(3):475-86. Epub 2005 Apr 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15878175 15878175]
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Detachment of Actinobacillus actinomycetemcomitans biofilm cells by an endogenous beta-hexosaminidase activity., Kaplan JB, Ragunath C, Ramasubbu N, Fine DH, J Bacteriol. 2003 Aug;185(16):4693-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12896987 12896987]
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Enzymatic detachment of Staphylococcus epidermidis biofilms., Kaplan JB, Ragunath C, Velliyagounder K, Fine DH, Ramasubbu N, Antimicrob Agents Chemother. 2004 Jul;48(7):2633-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15215120 15215120]
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Genes involved in the synthesis and degradation of matrix polysaccharide in Actinobacillus actinomycetemcomitans and Actinobacillus pleuropneumoniae biofilms., Kaplan JB, Velliyagounder K, Ragunath C, Rohde H, Mack D, Knobloch JK, Ramasubbu N, J Bacteriol. 2004 Dec;186(24):8213-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15576769 15576769]
[[Category: Aggregatibacter actinomycetemcomitans]]
[[Category: Aggregatibacter actinomycetemcomitans]]
[[Category: Beta-N-acetylhexosaminidase]]
[[Category: Beta-N-acetylhexosaminidase]]
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[[Category: Thomas, L M.]]
[[Category: Thomas, L M.]]
[[Category: Beta barrel]]
[[Category: Beta barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:20:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:04:57 2008''

Revision as of 03:04, 28 July 2008

Image:1yht.png

Template:STRUCTURE 1yht

Crystal structure analysis of Dispersin B

Template:ABSTRACT PUBMED 15878175

About this Structure

1YHT is a Single protein structure of sequence from Aggregatibacter actinomycetemcomitans. Full crystallographic information is available from OCA.

Reference

Structural analysis of dispersin B, a biofilm-releasing glycoside hydrolase from the periodontopathogen Actinobacillus actinomycetemcomitans., Ramasubbu N, Thomas LM, Ragunath C, Kaplan JB, J Mol Biol. 2005 Jun 10;349(3):475-86. Epub 2005 Apr 14. PMID:15878175

Detachment of Actinobacillus actinomycetemcomitans biofilm cells by an endogenous beta-hexosaminidase activity., Kaplan JB, Ragunath C, Ramasubbu N, Fine DH, J Bacteriol. 2003 Aug;185(16):4693-8. PMID:12896987

Enzymatic detachment of Staphylococcus epidermidis biofilms., Kaplan JB, Ragunath C, Velliyagounder K, Fine DH, Ramasubbu N, Antimicrob Agents Chemother. 2004 Jul;48(7):2633-6. PMID:15215120

Genes involved in the synthesis and degradation of matrix polysaccharide in Actinobacillus actinomycetemcomitans and Actinobacillus pleuropneumoniae biofilms., Kaplan JB, Velliyagounder K, Ragunath C, Rohde H, Mack D, Knobloch JK, Ramasubbu N, J Bacteriol. 2004 Dec;186(24):8213-20. PMID:15576769

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