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| - | [[Image:1yjq.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:1yjq.png|left|200px]] |
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| | {{STRUCTURE_1yjq| PDB=1yjq | SCENE= }} | | {{STRUCTURE_1yjq| PDB=1yjq | SCENE= }} |
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| - | '''Crystal structure of ketopantoate reductase in complex with NADP+'''
| + | ===Crystal structure of ketopantoate reductase in complex with NADP+=== |
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| - | ==Overview==
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| - | The NADPH-dependent reduction of ketopantoate to pantoate, catalyzed by ketopantoate reductase (KPR; EC 1.1.1.169), is essential for the biosynthesis of pantothenate (vitamin B(5)). Here we present the crystal structure of Escherichia coli KPR with NADP(+) bound, solved to 2.1 A resolution. The cofactor is bound in the active site cleft between the N-terminal Rossmann-fold domain and the C-terminal alpha-helical domain. The thermodynamics of cofactor and substrate binding were characterized by isothermal titration calorimetry. The dissociation constant for NADP(+) was found to be 6.5 muM, 20-fold larger than that for NADPH (0.34 muM). The difference is primarily due to the entropic term, suggesting favorable hydrophobic interactions of the more lipophilic nicotinamide ring in NADPH. Comparison of this binary complex structure with the previously studied apoenzyme reveals no evidence for large domain movements on cofactor binding. This observation is further supported both by molecular dynamics and by calorimetric analysis. A model of the ternary complex, based on the structure presented here, provides novel insights into the molecular mechanism of enzyme catalysis. We propose a conformational switch of the essential Lys176 from the "resting" state observed in our structure to an "active" state, to bind ketopantoate. Additionally, we identify the importance of Asn98 for substrate binding and enzyme catalysis. | + | The line below this paragraph, {{ABSTRACT_PUBMED_15966718}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15966718 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15966718}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Pantothenate pathway]] | | [[Category: Pantothenate pathway]] |
| | [[Category: Secondary alcohol dehydrogenase]] | | [[Category: Secondary alcohol dehydrogenase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:24:25 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:41:40 2008'' |
Revision as of 20:41, 28 July 2008
Template:STRUCTURE 1yjq
Crystal structure of ketopantoate reductase in complex with NADP+
Template:ABSTRACT PUBMED 15966718
About this Structure
1YJQ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The crystal structure of Escherichia coli ketopantoate reductase with NADP+ bound., Lobley CM, Ciulli A, Whitney HM, Williams G, Smith AG, Abell C, Blundell TL, Biochemistry. 2005 Jun 28;44(25):8930-9. PMID:15966718
Page seeded by OCA on Mon Jul 28 23:41:40 2008
