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1ymh

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[[Image:1ymh.gif|left|200px]]
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{{STRUCTURE_1ymh| PDB=1ymh | SCENE= }}
{{STRUCTURE_1ymh| PDB=1ymh | SCENE= }}
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'''anti-HCV Fab 19D9D6 complexed with protein L (PpL) mutant A66W'''
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===anti-HCV Fab 19D9D6 complexed with protein L (PpL) mutant A66W===
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==Overview==
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Protein L from Peptostreptococcus magnus (PpL) is a multidomain protein composed of four or five immunoglobulin-binding domains that target the kappa light chain of a large repertoire of human and murine antibodies. Thus, a single domain of this protein can be used to aid the crystallization of Fab, free or complexed to their antigen when it is not possible to obtain crystals without it. Each wild-type PpL domain has two light-chain binding sites that target the same region of the light chain and can thus bring together two Fab-antigen complexes within the crystal lattice. In this context the small PpL domain is sandwiched between two Fab and cannot participate in crystal contacts, thus mutants are unlikely to increase the chances of crystallizing a particular complex. However, it is possible to design mutants that can bind at only one site by making use of the crystal structures obtained so far. Such mutants will have a free surface that can participate in crystal contacts and that can be modified to improve its crystal contact-forming properties. Here, a comparison of two single-site mutants that differ at three different positions is reported. In both mutants two different tryptophan residues participate in crystal-packing interactions, suggesting that this residue may be particularly interesting for enhancing crystal-contact formation.
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The line below this paragraph, {{ABSTRACT_PUBMED_15930633}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15930633 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15930633}}
==About this Structure==
==About this Structure==
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[[Category: Engineering of crystal contact]]
[[Category: Engineering of crystal contact]]
[[Category: Ppl-fab complex]]
[[Category: Ppl-fab complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:30:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:27:51 2008''

Revision as of 02:28, 29 July 2008

Image:1ymh.png

Template:STRUCTURE 1ymh

anti-HCV Fab 19D9D6 complexed with protein L (PpL) mutant A66W

Template:ABSTRACT PUBMED 15930633

About this Structure

Full crystallographic information is available from OCA.

Reference

Comparison of the crystallization and crystal packing of two Fab single-site mutant protein L complexes., Granata V, Housden NG, Harrison S, Jolivet-Reynaud C, Gore MG, Stura EA, Acta Crystallogr D Biol Crystallogr. 2005 Jun;61(Pt 6):750-4. Epub 2005, May 26. PMID:15930633

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