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1yqd

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{{STRUCTURE_1yqd| PDB=1yqd | SCENE= }}
{{STRUCTURE_1yqd| PDB=1yqd | SCENE= }}
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'''Sinapyl Alcohol Dehydrogenase complexed with NADP+'''
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===Sinapyl Alcohol Dehydrogenase complexed with NADP+===
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==Overview==
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We describe the three-dimensional structure of sinapyl alcohol dehydrogenase (SAD) from Populus tremuloides (aspen), a member of the NADP(H)-dependent dehydrogenase family that catalyzes the last reductive step in the formation of monolignols. The active site topology revealed by the crystal structure substantiates kinetic results indicating that SAD maintains highest specificity for the substrate sinapaldehyde. We also report substantial substrate inhibition kinetics for the SAD-catalyzed reduction of hydroxycinnamaldehydes. Although SAD and classical cinnamyl alcohol dehydrogenases (CADs) catalyze the same reaction and share some sequence identity, the active site topology of SAD is strikingly different from that predicted for classical CADs. Kinetic analyses of wild-type SAD and several active site mutants demonstrate the complexity of defining determinants of substrate specificity in these enzymes. These results, along with a phylogenetic analysis, support the inclusion of SAD in a plant alcohol dehydrogenase subfamily that includes cinnamaldehyde and benzaldehyde dehydrogenases. We used the SAD three-dimensional structure to model several of these SAD-like enzymes, and although their active site topologies largely mirror that of SAD, we describe a correlation between substrate specificity and amino acid substitution patterns in their active sites. The SAD structure thus provides a framework for understanding substrate specificity in this family of enzymes and for engineering new enzyme specificities.
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(as it appears on PubMed at http://www.pubmed.gov), where 15829607 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15829607}}
==About this Structure==
==About this Structure==
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[[Category: Substrate inhibition]]
[[Category: Substrate inhibition]]
[[Category: Zinc-dependent]]
[[Category: Zinc-dependent]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:38:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:30:05 2008''

Revision as of 16:30, 27 July 2008

Image:1yqd.png

Template:STRUCTURE 1yqd

Sinapyl Alcohol Dehydrogenase complexed with NADP+

Template:ABSTRACT PUBMED 15829607

About this Structure

1YQD is a Single protein structure of sequence from Populus tremuloides. Full crystallographic information is available from OCA.

Reference

Structural and kinetic basis for substrate selectivity in Populus tremuloides sinapyl alcohol dehydrogenase., Bomati EK, Noel JP, Plant Cell. 2005 May;17(5):1598-611. Epub 2005 Apr 13. PMID:15829607

Page seeded by OCA on Sun Jul 27 19:30:05 2008

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