1ysl

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{{STRUCTURE_1ysl| PDB=1ysl | SCENE= }}
{{STRUCTURE_1ysl| PDB=1ysl | SCENE= }}
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'''Crystal structure of HMG-CoA synthase from Enterococcus faecalis with AcetoAcetyl-CoA ligand.'''
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===Crystal structure of HMG-CoA synthase from Enterococcus faecalis with AcetoAcetyl-CoA ligand.===
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==Overview==
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Biosynthesis of the isoprenoid precursor, isopentenyl diphosphate, is a critical function in all independently living organisms. There are two major pathways for this synthesis, the non-mevalonate pathway found in most eubacteria and the mevalonate pathway found in animal cells and a number of pathogenic bacteria. An early step in this pathway is the condensation of acetyl-CoA and acetoacetyl-CoA into HMG-CoA, catalyzed by the enzyme HMG-CoA synthase. To explore the possibility of a small molecule inhibitor of the enzyme functioning as a non-cell wall antibiotic, the structure of HMG-CoA synthase from Enterococcus faecalis (MVAS) was determined by selenomethionine MAD phasing to 2.4 A and the enzyme complexed with its second substrate, acetoacetyl-CoA, to 1.9 A. These structures show that HMG-CoA synthase from Enterococcus is a member of the family of thiolase fold enzymes and, while similar to the recently published HMG-CoA synthase structures from Staphylococcus aureus, exhibit significant differences in the structure of the C-terminal domain. The acetoacetyl-CoA binary structure demonstrates reduced coenzyme A and acetoacetate covalently bound to the active site cysteine through a thioester bond. This is consistent with the kinetics of the reaction that have shown acetoacetyl-CoA to be a potent inhibitor of the overall reaction, and provides a starting point in the search for a small molecule inhibitor.
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(as it appears on PubMed at http://www.pubmed.gov), where 16245942 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16245942}}
==About this Structure==
==About this Structure==
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[[Category: Coenzymea]]
[[Category: Coenzymea]]
[[Category: Thiolase family]]
[[Category: Thiolase family]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:43:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:13:25 2008''

Revision as of 05:13, 29 July 2008

Image:1ysl.png

Template:STRUCTURE 1ysl

Crystal structure of HMG-CoA synthase from Enterococcus faecalis with AcetoAcetyl-CoA ligand.

Template:ABSTRACT PUBMED 16245942

About this Structure

1YSL is a Single protein structure of sequence from Enterococcus faecalis. Full crystallographic information is available from OCA.

Reference

X-ray crystal structures of HMG-CoA synthase from Enterococcus faecalis and a complex with its second substrate/inhibitor acetoacetyl-CoA., Steussy CN, Vartia AA, Burgner JW 2nd, Sutherlin A, Rodwell VW, Stauffacher CV, Biochemistry. 2005 Nov 1;44(43):14256-67. PMID:16245942

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