1yth

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{{STRUCTURE_1yth| PDB=1yth | SCENE= }}
{{STRUCTURE_1yth| PDB=1yth | SCENE= }}
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'''SIV PROTEASE CRYSTALLIZED WITH PEPTIDE PRODUCT'''
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===SIV PROTEASE CRYSTALLIZED WITH PEPTIDE PRODUCT===
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==Overview==
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Strain is eliminated as a factor in hydrolysis of the scissile peptide bond by human immunodeficiency virus (HIV)-1 and simian immunodeficiency virus (SIV), based on the first eight complexes of products of hydrolysis with the enzymes. The carboxyl group generated at the scissile bond interacts with both catalytic aspartic acids. The structures directly suggest the interactions of the gemdiol intermediate with the active site. Based on the structures, the nucleophilic water is displaced stereospecifically by substrate binding toward one catalytic aspartic acid, while the scissile carbonyl becomes hydrogen bonded to the other catalytic aspartic acid in position for hydrolysis. Crystal structures for two N-terminal (P) products and two C-terminal (Q) products provide unambiguous density for the ligands at 2.2-2.6 A resolution and 17-21% R factors. The N-terminal product, Ac-S-L-N-F/, overlaps closely with the N-terminal sequences of peptidomimetic inhibitors bound to the protease. Comparison of the two C-terminal products, /F-L-E-K and /F(NO2)-E-A-Nle-S, indicates that the P2' residue is highly constrained, while the positioning of the P1' and P3' residues are sequence dependent.
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The line below this paragraph, {{ABSTRACT_PUBMED_8841139}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 8841139 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8841139}}
==About this Structure==
==About this Structure==
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[[Category: Polyprotein]]
[[Category: Polyprotein]]
[[Category: Rna-directed dna polymerase]]
[[Category: Rna-directed dna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:46:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:20:20 2008''

Revision as of 13:20, 29 July 2008

Image:1yth.png

Template:STRUCTURE 1yth

SIV PROTEASE CRYSTALLIZED WITH PEPTIDE PRODUCT

Template:ABSTRACT PUBMED 8841139

About this Structure

1YTH is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Three-dimensional structures of HIV-1 and SIV protease product complexes., Rose RB, Craik CS, Douglas NL, Stroud RM, Biochemistry. 1996 Oct 1;35(39):12933-44. PMID:8841139

Page seeded by OCA on Tue Jul 29 16:20:20 2008

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