1yxh

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{{STRUCTURE_1yxh| PDB=1yxh | SCENE= }}
{{STRUCTURE_1yxh| PDB=1yxh | SCENE= }}
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'''Crystal structure of a novel phospholipase A2 from Naja naja sagittifera with a strong anticoagulant activity'''
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===Crystal structure of a novel phospholipase A2 from Naja naja sagittifera with a strong anticoagulant activity===
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==Overview==
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This is the first PLA(2) crystal structure from group I that shows a strong anticoagulant property. The monomeric PLA(2) was purified from the venom of Naja naja sagittifera (Indian cobra). Its amino acid sequence has been determined using cDNA technique. The amino acid sequence of sPLA(2) contains three positively charged and two negatively charged residues in the segment 54-71 (numbering scheme of sPLA(2)) thus giving this region an overall cationic amphiphilic surface. This suggested the presence of an anticoagulant activity in sPLA(2). The enzyme was crystallized using hanging drop vapour diffusion method in the presence of calcium chloride. The crystals belong to space group P4(1) with cell dimensions of a=b=42.0A, c=65.9A. The X-ray crystal structure was determined at 1.8A resolution using molecular replacement method and refined to an R value of 0.179 for 10,023 reflections. The overall scaffolding of sPLA(2) is essentially similar to those observed for other group I PLA(2)s. However, the conformations of various surface loops were found to be significantly different. The most significant observation pertains to the anticoagulant loop in which both the acidic residues are engaged in intramolecular interactions whereas all the three basic residues are free to interact with other molecules. This makes the sPLA(2) a potentially strong anticoagulating molecule.
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(as it appears on PubMed at http://www.pubmed.gov), where 16269164 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16269164}}
==About this Structure==
==About this Structure==
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[[Category: Monomer]]
[[Category: Monomer]]
[[Category: Pla2]]
[[Category: Pla2]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:48:30 2008''

Revision as of 07:48, 28 July 2008

Image:1yxh.png

Template:STRUCTURE 1yxh

Crystal structure of a novel phospholipase A2 from Naja naja sagittifera with a strong anticoagulant activity

Template:ABSTRACT PUBMED 16269164

About this Structure

1YXH is a Single protein structure of sequence from Naja sagittifera. Full crystallographic information is available from OCA.

Reference

Crystal structure of a novel phospholipase A2 from Naja naja sagittifera with a strong anticoagulant activity., Jabeen T, Singh N, Singh RK, Ethayathulla AS, Sharma S, Srinivasan A, Singh TP, Toxicon. 2005 Dec 15;46(8):865-75. Epub 2005 Nov 2. PMID:16269164

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