1yxn

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[[Image:1yxn.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1yxn| PDB=1yxn | SCENE= }}
{{STRUCTURE_1yxn| PDB=1yxn | SCENE= }}
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'''Pseudo-atomic model of a fiberless isometric variant of bacteriophage phi29'''
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===Pseudo-atomic model of a fiberless isometric variant of bacteriophage phi29===
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==Overview==
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Bacteriophage phi29 is one of the smallest and simplest known dsDNA phages, making it amenable to structural investigations. The three-dimensional structure of a fiberless, isometric variant has been determined to 7.9 A resolution by cryo-electron microscopy (cryo-EM), allowing the identification of alpha helices and beta sheets. Their arrangement indicates that the folds of the phi29 and bacteriophage HK97 capsid proteins are similar except for an additional immunoglobulin-like domain of the phi29 protein. An atomic model that incorporates these two domains fits well into the cryo-EM density of the T = 3, fiberless isometric phi29 particle, and cryo-EM structures of fibered isometric and fiberless prolate prohead phi29 particles at resolutions of 8.7 A and 12.7 A, respectively. Thus, phi29 joins the growing number of phages that utilize the HK97 capsid structure, suggesting that this protein fold may be as prevalent in capsids of dsDNA phages as the jelly roll fold is in eukaryotic viruses.
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The line below this paragraph, {{ABSTRACT_PUBMED_15837419}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15837419 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15837419}}
==About this Structure==
==About this Structure==
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[[Category: Phage]]
[[Category: Phage]]
[[Category: Phi29]]
[[Category: Phi29]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:55:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 18:44:33 2008''

Revision as of 15:44, 28 July 2008

Image:1yxn.png

Template:STRUCTURE 1yxn

Pseudo-atomic model of a fiberless isometric variant of bacteriophage phi29

Template:ABSTRACT PUBMED 15837419

About this Structure

Full crystallographic information is available from OCA.

Reference

Conservation of the capsid structure in tailed dsDNA bacteriophages: the pseudoatomic structure of phi29., Morais MC, Choi KH, Koti JS, Chipman PR, Anderson DL, Rossmann MG, Mol Cell. 2005 Apr 15;18(2):149-59. PMID:15837419

Page seeded by OCA on Mon Jul 28 18:44:33 2008

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