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1z3m

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[[Image:1z3m.gif|left|200px]]
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{{STRUCTURE_1z3m| PDB=1z3m | SCENE= }}
{{STRUCTURE_1z3m| PDB=1z3m | SCENE= }}
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'''Crystal structure of mutant Ribonuclease S (F8Nva)'''
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===Crystal structure of mutant Ribonuclease S (F8Nva)===
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==Overview==
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While the hydrophobic driving force is thought to be a major contributor to protein stability, it is difficult to experimentally dissect out its contribution to the overall free energy of folding. We have made large to small substitutions of buried hydrophobic residues at positions 8 and 13 in the peptide/protein complex, RNase-S, and have characterized the structures by X-ray crystallography. The thermodynamics of association of these mutant S peptides with S protein was measured in the presence of different concentrations of methanol and ethanol. The reduction in the strength of the hydrophobic driving force in the presence of these organic solvents was estimated from surface-tension data as well as from the dependence of the DeltaC(p) of protein/peptide binding on the alcohol concentration. The data indicated a decrease in the strength of the hydrophobic driving force of about 30-40% over a 0-30% range of the alcohol concentration. We observe that large to small substitutions destabilize the protein. However, the amount of destabilization, relative to the wild type, is independent of the alcohol concentration over the range of alcohol concentrations studied. The data clearly indicate that decreased stability of the mutants is primarily due to the loss of packing interactions rather than a reduced hydrophobic driving force and suggest a value of the hydrophobic driving force of less than 18 cal mol(-)(1) A(2).
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(as it appears on PubMed at http://www.pubmed.gov), where 15823052 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15823052}}
==About this Structure==
==About this Structure==
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[[Category: S-peptide]]
[[Category: S-peptide]]
[[Category: S-protein]]
[[Category: S-protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:08:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:52:31 2008''

Revision as of 23:52, 28 July 2008

Image:1z3m.png

Template:STRUCTURE 1z3m

Crystal structure of mutant Ribonuclease S (F8Nva)

Template:ABSTRACT PUBMED 15823052

About this Structure

1Z3M is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

Reference

Attempts to delineate the relative contributions of changes in hydrophobicity and packing to changes in stability of ribonuclease S mutants., Das M, Rao BV, Ghosh S, Varadarajan R, Biochemistry. 2005 Apr 19;44(15):5923-30. PMID:15823052

Page seeded by OCA on Tue Jul 29 02:52:31 2008

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