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1z4o

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[[Image:1z4o.gif|left|200px]]
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{{Seed}}
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[[Image:1z4o.png|left|200px]]
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{{STRUCTURE_1z4o| PDB=1z4o | SCENE= }}
{{STRUCTURE_1z4o| PDB=1z4o | SCENE= }}
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'''Structure of beta-phosphoglucomutase with inhibitor bound alpha-galactose 1-phosphate'''
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===Structure of beta-phosphoglucomutase with inhibitor bound alpha-galactose 1-phosphate===
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==Overview==
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This communication reports the X-ray crystal structure of the alpha-d-galactose-1-phosphate complex with that of Lactococcus lactis beta-phosphoglucomutase (beta-PGM) crystallized in the presence of Mg2+ cofactor and the enzyme-to-phosphorus ratio determined by protein and phosphate analyses of the crystalline complex. The 1:1 ratio determined for this complex was compared to the 1:2 ratio determined for the crystals of beta-PGM grown in the presence of substrate and Mg2+ cofactor. This result verifies the published structure assignment of this latter complex as the phosphorane adduct formed by covalent bonding between the active site Asp8 carboxylate to the C(1)phosphorus of the beta-glucose 1,6-bisphosphate ligand and rules out the proposal of a beta-PGM-glucose-6-phosphate-1-MgF3- complex.
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The line below this paragraph, {{ABSTRACT_PUBMED_15826149}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15826149 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15826149}}
==About this Structure==
==About this Structure==
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[[Category: Beta-phosphoglucomutase]]
[[Category: Beta-phosphoglucomutase]]
[[Category: Isomerase]]
[[Category: Isomerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:10:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:30:30 2008''

Revision as of 14:30, 28 July 2008

Image:1z4o.png

Template:STRUCTURE 1z4o

Structure of beta-phosphoglucomutase with inhibitor bound alpha-galactose 1-phosphate

Template:ABSTRACT PUBMED 15826149

About this Structure

1Z4O is a Single protein structure of sequence from Lactococcus lactis. Full crystallographic information is available from OCA.

Reference

Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase., Tremblay LW, Zhang G, Dai J, Dunaway-Mariano D, Allen KN, J Am Chem Soc. 2005 Apr 20;127(15):5298-9. PMID:15826149

Page seeded by OCA on Mon Jul 28 17:30:30 2008

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