This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1z5u
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | [[Image:1z5u. | + | {{Seed}} |
| + | [[Image:1z5u.png|left|200px]] | ||
<!-- | <!-- | ||
| Line 9: | Line 10: | ||
{{STRUCTURE_1z5u| PDB=1z5u | SCENE= }} | {{STRUCTURE_1z5u| PDB=1z5u | SCENE= }} | ||
| - | + | ===Crystal structure of S. typhimurium AphA complexed with cyclic-AMP=== | |
| - | |||
| - | ==Overview== | ||
| - | The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA protein was purified to homogeneity. The protein crystallized in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent content of approximately 54%. The crystals are composed of biologically active AphA molecules. | ||
==About this Structure== | ==About this Structure== | ||
| - | 1Z5U is a | + | 1Z5U is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z5U OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:14501135</ref><references group="xtra"/> | |
[[Category: Acid phosphatase]] | [[Category: Acid phosphatase]] | ||
[[Category: Salmonella typhimurium]] | [[Category: Salmonella typhimurium]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Kumar, V.]] | [[Category: Kumar, V.]] | ||
[[Category: Makde, R D.]] | [[Category: Makde, R D.]] | ||
| Line 28: | Line 25: | ||
[[Category: Cyclic-amp complex of apha protein]] | [[Category: Cyclic-amp complex of apha protein]] | ||
[[Category: Metalloenzyme]] | [[Category: Metalloenzyme]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 13:27:26 2009'' | ||
Revision as of 11:27, 17 February 2009
Crystal structure of S. typhimurium AphA complexed with cyclic-AMP
About this Structure
1Z5U is a 4 chains structure of sequences from Salmonella typhimurium. Full crystallographic information is available from OCA.
Reference
- Makde RD, Kumar V, Gupta GD, Jasti J, Singh TP, Mahajan SK. Expression, purification, crystallization and preliminary X-ray diffraction studies of recombinant class B non-specific acid phosphatase of Salmonella typhimurium. Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1849-52. Epub 2003, Sep 19. PMID:14501135
Page seeded by OCA on Tue Feb 17 13:27:26 2009
