This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1z9h

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1z9h.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1z9h.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1z9h| PDB=1z9h | SCENE= }}
{{STRUCTURE_1z9h| PDB=1z9h | SCENE= }}
-
'''Microsomal prostaglandin E synthase type-2'''
+
===Microsomal prostaglandin E synthase type-2===
-
==Overview==
+
<!--
-
Prostaglandin (PG) H(2) (PGH(2)), formed from arachidonic acid, is an unstable intermediate and is converted efficiently into more stable arachidonate metabolites (PGD(2), PGE(2), and PGF(2)) by the action of three groups of enzymes. Prostaglandin E synthase catalyzes an isomerization reaction, PGH(2) to PGE(2). Microsomal prostaglandin E synthase type-2 (mPGES-2) has been crystallized with an anti-inflammatory drug indomethacin (IMN), and the complex structure has been determined at 2.6A resolution. mPGES-2 forms a dimer and is attached to lipid membrane by anchoring the N-terminal section. Two hydrophobic pockets connected to form a V shape are located in the bottom of a large cavity. IMN binds deeply in the cavity by placing the OMe-indole and chlorophenyl moieties into the V-shaped pockets, respectively, and the carboxyl group interacts with S(gamma) of C110 by forming a H-bond. A characteristic H-bond chain formation (N-H...S(gamma)-H...S(gamma)...H-N) is seen through Y107-C113-C110-F112, which apparently decreases the pK(a) of S(gamma) of C110. The geometry suggests that the S(gamma) of C110 is most likely the catalytic site of mPGES-2. A search of the RCSB Protein Data Bank suggests that IMN can fit into the PGH(2) binding site in various proteins. On the basis of the crystal structure and mutation data, a PGH(2)-bound model structure was built. PGH(2) fits well into the IMN binding site by placing the alpha and omega-chains in the V-shaped pockets, and the endoperoxide moiety interacts with S(gamma) of C110. A possible catalytic mechanism is proposed on the basis of the crystal and model structures, and an alternative catalytic mechanism is described. The fold of mPGES-2 is quite similar to those of GSH-dependent hematopoietic prostaglandin D synthase, except for the two large loop sections.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15854652}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15854652 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15854652}}
==About this Structure==
==About this Structure==
Line 31: Line 35:
[[Category: Membran associated protein]]
[[Category: Membran associated protein]]
[[Category: Prostaglandin e synthase]]
[[Category: Prostaglandin e synthase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:20:55 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:34:49 2008''

Revision as of 03:34, 29 July 2008

Image:1z9h.png

Template:STRUCTURE 1z9h

Microsomal prostaglandin E synthase type-2

Template:ABSTRACT PUBMED 15854652

About this Structure

1Z9H is a Single protein structure of sequence from Macaca fascicularis. Full crystallographic information is available from OCA.

Reference

Crystal structure and possible catalytic mechanism of microsomal prostaglandin E synthase type 2 (mPGES-2)., Yamada T, Komoto J, Watanabe K, Ohmiya Y, Takusagawa F, J Mol Biol. 2005 May 20;348(5):1163-76. Epub 2005 Apr 2. PMID:15854652

Page seeded by OCA on Tue Jul 29 06:34:49 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1z9h"
Personal tools