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1zdc

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{{STRUCTURE_1zdc| PDB=1zdc | SCENE= }}
{{STRUCTURE_1zdc| PDB=1zdc | SCENE= }}
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'''DISULFIDE-STABILIZED MINI PROTEIN A DOMAIN, Z34C, NMR, 24 STRUCTURES'''
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===DISULFIDE-STABILIZED MINI PROTEIN A DOMAIN, Z34C, NMR, 24 STRUCTURES===
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==Overview==
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The affinity between molecules depends both on the nature and presentation of the contacts. Here, we observe coupling of functional and structural elements when a protein binding domain is evolved to a smaller functional mimic. Previously, a 38-residue form of the 59-residue B-domain of protein A, termed Z38, was selected by phage display. Z38 contains 13 mutations and binds IgG only 10-fold weaker than the native B-domain. We present the solution structure of Z38 and show that it adopts a tertiary structure remarkably similar to that observed for the first two helices of B-domain in the B-domain/Fc complex [Deisenhofer, J. (1981) Biochemistry 20, 2361-2370], although it is significantly less stable. Based on this structure, we have improved on Z38 by designing a 34-residue disulfide-bonded variant (Z34C) that has dramatically enhanced stability and binds IgG with 9-fold higher affinity. The improved stability of Z34C led to NMR spectra with much greater chemical shift dispersion, resulting in a more precisely determined structure. Z34C, like Z38, has a structure virtually identical to the equivalent region from native protein A domains. The well-defined hydrophobic core of Z34C reveals key structural features that have evolved in this small, functional domain. Thus, the stabilized two-helix peptide, about half the size and having one-third of the remaining residues altered, accurately mimics both the structure and function of the native domain.
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(as it appears on PubMed at http://www.pubmed.gov), where 9294166 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9294166}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZDC OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZDC OCA].
==Reference==
==Reference==
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[[Category: Igg binding domain]]
[[Category: Igg binding domain]]
[[Category: Protein a mimic]]
[[Category: Protein a mimic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:29:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:09:46 2008''

Revision as of 10:09, 27 July 2008

Image:1zdc.png

Template:STRUCTURE 1zdc

DISULFIDE-STABILIZED MINI PROTEIN A DOMAIN, Z34C, NMR, 24 STRUCTURES

Template:ABSTRACT PUBMED 9294166

About this Structure

Full experimental information is available from OCA.

Reference

Structural mimicry of a native protein by a minimized binding domain., Starovasnik MA, Braisted AC, Wells JA, Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10080-5. PMID:9294166

Page seeded by OCA on Sun Jul 27 13:09:46 2008

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