1zem

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{{STRUCTURE_1zem| PDB=1zem | SCENE= }}
{{STRUCTURE_1zem| PDB=1zem | SCENE= }}
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'''Crystal Structure of NAD+-Bound Xylitol Dehydrogenase'''
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===Crystal Structure of NAD+-Bound Xylitol Dehydrogenase===
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==Overview==
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Xylitol dehydrogenase (XDH) is one of several enzymes responsible for assimilating xylose into eukaryotic metabolism and is useful for fermentation of xylose contained in agricultural byproducts to produce ethanol. For efficient xylose utilization at high flux rates, cosubstrates should be recycled between the NAD+-specific XDH and the NADPH-preferring xylose reductase, another enzyme in the pathway. To understand and alter the cosubstrate specificity of XDH, we determined the crystal structure of the Gluconobacter oxydans holoenzyme to 1.9 angstroms resolution. The structure reveals that NAD+ specificity is largely conferred by Asp38, which interacts with the hydroxyls of the adenosine ribose. Met39 stacked under the purine ring and was also located near the 2' hydroxyl. Based on the location of these residues and on sequence alignments with related enzymes of various cosubstrate specificities, we constructed a double mutant (D38S/M39R) that was able to exclusively use NADP+, with no loss of activity.
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(as it appears on PubMed at http://www.pubmed.gov), where 16531240 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16531240}}
==About this Structure==
==About this Structure==
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[[Category: Dinucleotide-binding domain]]
[[Category: Dinucleotide-binding domain]]
[[Category: Rossmann fold]]
[[Category: Rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:31:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:19:57 2008''

Revision as of 02:20, 28 July 2008

Image:1zem.png

Template:STRUCTURE 1zem

Crystal Structure of NAD+-Bound Xylitol Dehydrogenase

Template:ABSTRACT PUBMED 16531240

About this Structure

1ZEM is a Single protein structure of sequence from Gluconobacter oxydans. Full crystallographic information is available from OCA.

Reference

Structure-guided engineering of xylitol dehydrogenase cosubstrate specificity., Ehrensberger AH, Elling RA, Wilson DK, Structure. 2006 Mar;14(3):567-75. PMID:16531240

Page seeded by OCA on Mon Jul 28 05:19:57 2008

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