1zja

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{{STRUCTURE_1zja| PDB=1zja | SCENE= }}
{{STRUCTURE_1zja| PDB=1zja | SCENE= }}
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'''Crystal structure of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45 (triclinic form)'''
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===Crystal structure of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45 (triclinic form)===
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==Overview==
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Various diseases related to the overconsumption of sugar make a growing need for sugar substitutes. Because sucrose is an inexpensive and readily available d-glucose donor, the industrial potential for enzymatic synthesis of the sucrose isomers trehalulose and/or isomaltulose from sucrose is large. The product specificity of sucrose isomerases that catalyze this reaction depends essentially on the possibility for tautomerization of sucrose, which is required for trehalulose formation. For optimal use of the enzyme, targeting controlled synthesis of these functional isomers, it is necessary to minimize the side reactions. This requires an extensive analysis of substrate binding modes and of the specificity-determining sites in the structure. The 1.6-2.2-A resolution three-dimensional structures of native and mutant complexes of a trehalulose synthase from Pseudomonas mesoacidophila MX-45 mimic successive states of the enzyme reaction. Combined with mutagenesis studies they give for the first time thorough insights into substrate recognition and processing and reaction specificities of these enzymes. Among the important outcomes of this study is the revelation of an aromatic clamp defined by Phe(256) and Phe(280) playing an essential role in substrate recognition and in controlling the reaction specificity, which is further supported by mutagenesis studies. Furthermore, this study highlights essential residues for binding the glucosyl and fructosyl moieties. The introduction of subtle changes informed by comparative three-dimensional structural data observed within our study can lead to fundamental modifications in the mode of action of sucrose isomerases and hence provide a template for industrial catalysts.
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(as it appears on PubMed at http://www.pubmed.gov), where 17597061 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17597061}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Trehalulose synthase native and carbohydrate complexed structures provide insights into sucrose isomerization., Ravaud S, Robert X, Watzlawick H, Haser R, Mattes R, Aghajari N, J Biol Chem. 2007 Sep 21;282(38):28126-36. Epub 2007 Jun 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17597061 17597061]
Trehalulose synthase native and carbohydrate complexed structures provide insights into sucrose isomerization., Ravaud S, Robert X, Watzlawick H, Haser R, Mattes R, Aghajari N, J Biol Chem. 2007 Sep 21;282(38):28126-36. Epub 2007 Jun 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17597061 17597061]
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Expression, purification, crystallization and preliminary X-ray crystallographic studies of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45., Ravaud S, Watzlawick H, Haser R, Mattes R, Aghajari N, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):100-3. Epub 2004 Dec 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16508103 16508103]
[[Category: Isomaltulose synthase]]
[[Category: Isomaltulose synthase]]
[[Category: Aghajari, N.]]
[[Category: Aghajari, N.]]
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[[Category: Sucrose isomerase]]
[[Category: Sucrose isomerase]]
[[Category: Trehalulose synthase]]
[[Category: Trehalulose synthase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:41:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 18:46:24 2008''

Revision as of 15:46, 28 July 2008

Image:1zja.png

Template:STRUCTURE 1zja

Crystal structure of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45 (triclinic form)

Template:ABSTRACT PUBMED 17597061

About this Structure

Full crystallographic information is available from OCA.

Reference

Trehalulose synthase native and carbohydrate complexed structures provide insights into sucrose isomerization., Ravaud S, Robert X, Watzlawick H, Haser R, Mattes R, Aghajari N, J Biol Chem. 2007 Sep 21;282(38):28126-36. Epub 2007 Jun 27. PMID:17597061

Expression, purification, crystallization and preliminary X-ray crystallographic studies of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45., Ravaud S, Watzlawick H, Haser R, Mattes R, Aghajari N, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):100-3. Epub 2004 Dec 2. PMID:16508103

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