2d1k
From Proteopedia
(New page: 200px<br /> <applet load="2d1k" size="450" color="white" frame="true" align="right" spinBox="true" caption="2d1k, resolution 2.50Å" /> '''Ternary complex of ...) |
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| - | [[Image:2d1k.gif|left|200px]]<br /> | + | [[Image:2d1k.gif|left|200px]]<br /><applet load="2d1k" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="2d1k, resolution 2.50Å" /> | caption="2d1k, resolution 2.50Å" /> | ||
'''Ternary complex of the WH2 domain of mim with actin-dnase I'''<br /> | '''Ternary complex of the WH2 domain of mim with actin-dnase I'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2D1K is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with CA, MG and ATP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Deoxyribonuclease_I Deoxyribonuclease I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.1 3.1.21.1] Full crystallographic information is available from [http:// | + | 2D1K is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ATP:'>ATP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Deoxyribonuclease_I Deoxyribonuclease I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.1 3.1.21.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: wip]] | [[Category: wip]] | ||
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Revision as of 13:14, 23 January 2008
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Ternary complex of the WH2 domain of mim with actin-dnase I
Overview
The adaptor protein missing-in-metastasis (MIM) contains independent F-, and G-actin binding domains, consisting, respectively, of an N-terminal, 250 aa IRSp53/MIM homology domain (IMD) and a C-terminal WASP-homology, domain 2 (WH2). We determined the crystal structures of MIM's IMD and that, of its WH2 bound to actin. The IMD forms a dimer, with each subunit folded, as an antiparallel three-helix bundle. This fold is related to that of the, BAR domain. Like the BAR domain, the IMD has been implicated in membrane, binding. Yet, comparison of the structures reveals that the membrane, binding surfaces of the two domains have opposite curvatures, which may, determine the type of curvature of the interacting membrane. The WH2 of, MIM is longer than the prototypical WH2, interacting with all four, subdomains of actin. We characterize a similar WH2 at the C terminus of, IRSp53 and propose that in these two proteins WH2 performs a scaffolding, function.
About this Structure
2D1K is a Protein complex structure of sequences from Bos taurus and Oryctolagus cuniculus with , and as ligands. Active as Deoxyribonuclease I, with EC number 3.1.21.1 Full crystallographic information is available from OCA.
Reference
Structural basis for the actin-binding function of missing-in-metastasis., Lee SH, Kerff F, Chereau D, Ferron F, Klug A, Dominguez R, Structure. 2007 Feb;15(2):145-55. PMID:17292833
Page seeded by OCA on Wed Jan 23 15:14:21 2008
Categories: Bos taurus | Deoxyribonuclease I | Oryctolagus cuniculus | Protein complex | Chereau, D. | Dominguez, R. | Kerff, F. | ATP | CA | MG | Actin | Dnase i | Wasp | Wh2 | Wip
