1zm7

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1zm7.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1zm7.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1zm7| PDB=1zm7 | SCENE= }}
{{STRUCTURE_1zm7| PDB=1zm7 | SCENE= }}
-
'''Crystal structure of D. melanogaster deoxyribonucleoside kinase mutant N64D in complex with dTTP'''
+
===Crystal structure of D. melanogaster deoxyribonucleoside kinase mutant N64D in complex with dTTP===
-
==Overview==
+
<!--
-
The Drosophila melanogaster deoxyribonucleoside kinase (Dm-dNK) double mutant N45D/N64D was identified during a previous directed evolution study. This mutant enzyme had a decreased activity towards the natural substrates and decreased feedback inhibition with dTTP, whereas the activity with 3'-modified nucleoside analogs like 3'-azidothymidine (AZT) was nearly unchanged. Here, we identify the mutation N64D as being responsible for these changes. Furthermore, we crystallized the mutant enzyme in the presence of one of its substrates, thymidine, and the feedback inhibitor, dTTP. The introduction of the charged Asp residue appears to destabilize the LID region (residues 167-176) of the enzyme by electrostatic repulsion and no hydrogen bond to the 3'-OH is made in the substrate complex by Glu172 of the LID region. This provides a binding space for more bulky 3'-substituents like the azido group in AZT but influences negatively the interactions between Dm-dNK, substrates and feedback inhibitors based on deoxyribose. The detailed picture of the structure-function relationship provides an improved background for future development of novel mutant suicide genes for Dm-dNK-mediated gene therapy.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16008571}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16008571 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16008571}}
==About this Structure==
==About this Structure==
Line 34: Line 38:
[[Category: Mutant]]
[[Category: Mutant]]
[[Category: N64d]]
[[Category: N64d]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:47:59 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:31:19 2008''

Revision as of 05:31, 28 July 2008

Image:1zm7.png

Template:STRUCTURE 1zm7

Crystal structure of D. melanogaster deoxyribonucleoside kinase mutant N64D in complex with dTTP

Template:ABSTRACT PUBMED 16008571

About this Structure

1ZM7 is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

Reference

Structural basis for the changed substrate specificity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D., Welin M, Skovgaard T, Knecht W, Zhu C, Berenstein D, Munch-Petersen B, Piskur J, Eklund H, FEBS J. 2005 Jul;272(14):3733-42. PMID:16008571

Page seeded by OCA on Mon Jul 28 08:31:19 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1zm7"
Personal tools