1zmz

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[[Image:1zmz.gif|left|200px]]
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{{STRUCTURE_1zmz| PDB=1zmz | SCENE= }}
{{STRUCTURE_1zmz| PDB=1zmz | SCENE= }}
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'''Solution structure of the N-terminal domain (M1-S98) of human centrin 2'''
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===Solution structure of the N-terminal domain (M1-S98) of human centrin 2===
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==Overview==
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Centrins are well-conserved calcium binding proteins from the EF-hand superfamily implicated in various cellular functions, such as centrosome duplication, DNA repair, and nuclear mRNA export. The intrinsic molecular flexibility and the self-association tendency make difficult the structural characterization of the integral protein. In this paper we report the solution structure, the Ca2+ binding properties, and the intermolecular interactions of the N-terminal domain of two human centrin isoforms, HsCen1 and HsCen2. In the absence of Ca2+, the N-terminal construct of HsCen2 revealed a compact core conformation including four almost antiparallel alpha-helices and a short antiparallel beta-sheet, very similar to the apo state structure of other calcium regulatory EF-hand domains. The first 25 residues show a highly irregular and dynamic structure. The three-dimensional model for the N-terminal domain of HsCen1, based on the high sequence conservation and NMR spectroscopic data, shows very close structural properties. Ca2+ titration of the apo-N-terminal domain of HsCen1 and HsCen2, monitored by NMR spectroscopy, revealed a very weak affinity (10(2)-10(3) M(-1)), suggesting that the cellular role of this domain is not calcium dependent. Isothermal calorimetric titrations showed that an 18-residue peptide, derived from the N-terminal unstructured fragment, has a significant affinity (approximately 10(5) M(-1)) for the isolated C-terminal domain, suggesting an active role in the self-assembly of centrin molecules.
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(as it appears on PubMed at http://www.pubmed.gov), where 16411764 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16411764}}
==About this Structure==
==About this Structure==
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1ZMZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZMZ OCA].
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1ZMZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZMZ OCA].
==Reference==
==Reference==
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[[Category: Self-association]]
[[Category: Self-association]]
[[Category: Solution structure]]
[[Category: Solution structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:49:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:48:55 2008''

Revision as of 11:49, 28 July 2008

Image:1zmz.png

Template:STRUCTURE 1zmz

Solution structure of the N-terminal domain (M1-S98) of human centrin 2

Template:ABSTRACT PUBMED 16411764

About this Structure

1ZMZ is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

The N-terminal domain of human centrin 2 has a closed structure, binds calcium with a very low affinity, and plays a role in the protein self-assembly., Yang A, Miron S, Duchambon P, Assairi L, Blouquit Y, Craescu CT, Biochemistry. 2006 Jan 24;45(3):880-9. PMID:16411764

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