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1zoi

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{{STRUCTURE_1zoi| PDB=1zoi | SCENE= }}
{{STRUCTURE_1zoi| PDB=1zoi | SCENE= }}
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'''Crystal Structure of a Stereoselective Esterase from Pseudomonas putida IFO12996'''
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===Crystal Structure of a Stereoselective Esterase from Pseudomonas putida IFO12996===
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==Overview==
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Esterase (EST) from Pseudomonas putida IFO12996 catalyzes the stereoselective hydrolysis of methyl dl-beta-acetylthioisobutyrate (dl-MATI) to produce d-beta-acetylthioisobutyric acid (DAT), serving as a key intermediate for the synthesis of angiotensin-converting enzyme inhibitors. The EST gene was cloned and expressed in Escherichia coli; the recombinant protein is a non-disulfide-linked homotrimer with a monomer molecular weight of 33,000 in both solution and crystalline states, indicating that these ESTs function as trimers. EST hydrolyzed dl-MATI to produce DAT with a degree of conversion of 49.5% and an enantiomeric excess value of 97.2% at an optimum pH of about 8 to 10 and an optimum temperature of about 57 to 67 degrees C. The crystal structure of EST has been determined by X-ray diffraction to a resolution of 1.6 A, confirming that EST is a member of the alpha/beta hydrolase fold superfamily of enzymes and includes a catalytic triad of Ser97, Asp227, and His256. The active site is located approximately in the middle of the molecule at the end of a pocket approximately 12 A deep. EST can hydrolyze the methyl ester group without affecting the acetylthiol ester moiety in dl-MATI. The examination of substrate specificity of EST toward other linear esters revealed that the enzyme showed specific activity toward methyl esters and that it recognized the configuration at C-2.
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{{ABSTRACT_PUBMED_16321951}}
==About this Structure==
==About this Structure==
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[[Category: Esterase]]
[[Category: Esterase]]
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:52:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:22:34 2008''

Revision as of 14:22, 27 July 2008

Image:1zoi.png

Template:STRUCTURE 1zoi

Crystal Structure of a Stereoselective Esterase from Pseudomonas putida IFO12996

Template:ABSTRACT PUBMED 16321951

About this Structure

1ZOI is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

Stereoselective esterase from Pseudomonas putida IFO12996 reveals alpha/beta hydrolase folds for D-beta-acetylthioisobutyric acid synthesis., Elmi F, Lee HT, Huang JY, Hsieh YC, Wang YL, Chen YJ, Shaw SY, Chen CJ, J Bacteriol. 2005 Dec;187(24):8470-6. PMID:16321951

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