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1zsg

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[[Image:1zsg.gif|left|200px]]
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{{STRUCTURE_1zsg| PDB=1zsg | SCENE= }}
{{STRUCTURE_1zsg| PDB=1zsg | SCENE= }}
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'''beta PIX-SH3 complexed with an atypical peptide from alpha-PAK'''
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===beta PIX-SH3 complexed with an atypical peptide from alpha-PAK===
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==Overview==
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The PAK Ser/Thr kinases are important downstream effectors of the Rho family GTPases Cdc42 and Rac, partly mediating the role of these G proteins in cell proliferation and cytoskeletal rearrangements. As well as small G proteins, PAK interacts with the Cdc42/Rac exchange factor beta-PIX via the PIX SH3 domain and a nontypical Pro-rich region in PAK. This interaction is thought to affect the localization of PAK, as well as increased GTP/GDP exchange of Rac and Cdc42. We have determined the structure of the PIX-SH3/PAK peptide complex and shown that it differs from typical Src-like SH3/peptide complexes. The peptide makes contacts through the Pro-rich sequence in a similar way to standard SH3/peptide complexes, even though the Pro residue positions are not conserved. In addition, there are interactions with a Pro and Lys in the PAK, which are C-terminal to the conserved Arg found in all SH3-binding sequences. These contact a fourth binding pocket on the SH3 domain. We have measured the affinity of PIX-SH3 for the PAK peptide and found that it is of intermediate affinity. When PAK is activated, Ser-199 in the PIX-binding site is phosphorylated. This phosphorylation is sufficient to reduce the affinity for PIX 6-fold.
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(as it appears on PubMed at http://www.pubmed.gov), where 16101281 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16101281}}
==About this Structure==
==About this Structure==
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1ZSG is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZSG OCA].
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1ZSG is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZSG OCA].
==Reference==
==Reference==
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[[Category: Owen, D.]]
[[Category: Owen, D.]]
[[Category: Sh3-peptide complex]]
[[Category: Sh3-peptide complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:01:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:12:03 2008''

Revision as of 16:12, 28 July 2008

Image:1zsg.png

Template:STRUCTURE 1zsg

beta PIX-SH3 complexed with an atypical peptide from alpha-PAK

Template:ABSTRACT PUBMED 16101281

About this Structure

1ZSG is a Protein complex structure of sequences from Homo sapiens. Full experimental information is available from OCA.

Reference

Structural analysis of the SH3 domain of beta-PIX and its interaction with alpha-p21 activated kinase (PAK)., Mott HR, Nietlispach D, Evetts KA, Owen D, Biochemistry. 2005 Aug 23;44(33):10977-83. PMID:16101281

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