1zvq

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[[Image:1zvq.gif|left|200px]]
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{{Seed}}
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[[Image:1zvq.png|left|200px]]
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{{STRUCTURE_1zvq| PDB=1zvq | SCENE= }}
{{STRUCTURE_1zvq| PDB=1zvq | SCENE= }}
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'''Structure of the Q61G mutant of Ras in the GDP-bound form'''
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===Structure of the Q61G mutant of Ras in the GDP-bound form===
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==Overview==
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The flexibility of the conserved 57DTAGQ61 motif is essential for Ras proper cycling in response to growth factors. Here, we increase the flexibility of the 57DTAGQ61 motif by mutating Gln61 to Gly. The crystal structure of the RasQ61G mutant reveals a new conformation of switch 2 that bears remarkable structural homology to an intermediate for GTP hydrolysis revealed by targeted molecular dynamics simulations. The mutation increased retention of GTP and inhibited Ras binding to the catalytic site, but not to the distal site of Sos. Most importantly, the thermodynamics of RafRBD binding to Ras are altered even though the structure of switch 1 is not affected by the mutation. Our results suggest that interplay and transmission of structural information between the switch regions are important factors for Ras function. They propose that initiation of GTP hydrolysis sets off the separation of the Ras/effector complex even before the GDP conformation is reached.
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The line below this paragraph, {{ABSTRACT_PUBMED_16531227}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16531227 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16531227}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structure of a transient intermediate for GTP hydrolysis by ras., Ford B, Hornak V, Kleinman H, Nassar N, Structure. 2006 Mar;14(3):427-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16531227 16531227]
Structure of a transient intermediate for GTP hydrolysis by ras., Ford B, Hornak V, Kleinman H, Nassar N, Structure. 2006 Mar;14(3):427-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16531227 16531227]
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The structural basis for the transition from Ras-GTP to Ras-GDP., Hall BE, Bar-Sagi D, Nassar N, Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12138-42. Epub 2002 Sep 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12213964 12213964]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Gdp]]
[[Category: Gdp]]
[[Category: Gtpase]]
[[Category: Gtpase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:08:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:36:56 2008''

Revision as of 02:36, 28 July 2008

Image:1zvq.png

Template:STRUCTURE 1zvq

Structure of the Q61G mutant of Ras in the GDP-bound form

Template:ABSTRACT PUBMED 16531227

About this Structure

1ZVQ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of a transient intermediate for GTP hydrolysis by ras., Ford B, Hornak V, Kleinman H, Nassar N, Structure. 2006 Mar;14(3):427-36. PMID:16531227

The structural basis for the transition from Ras-GTP to Ras-GDP., Hall BE, Bar-Sagi D, Nassar N, Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12138-42. Epub 2002 Sep 4. PMID:12213964

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