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| - | [[Image:1zxi.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1zxi| PDB=1zxi | SCENE= }} | | {{STRUCTURE_1zxi| PDB=1zxi | SCENE= }} |
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| - | '''Reconstituted CO dehydrogenase from Oligotropha carboxidovorans'''
| + | ===Reconstituted CO dehydrogenase from Oligotropha carboxidovorans=== |
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| - | ==Overview==
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| - | Carbon monoxide dehydrogenase from the bacterium Oligotropha carboxidovorans catalyzes the oxidation of CO to CO(2) at a unique [CuSMoO(2)] cluster. In the bacteria the cluster is assembled post-translational. The integration of S, and particularly of Cu, is rate limiting in vivo, which leads to CO dehydrogenase preparations containing the mature and fully functional enzyme along with forms of the enzyme deficient in one or both of these elements. The active sites of mature and immature forms of CO dehydrogenase were converted into a [MoO(3)] centre by treatment with potassium cyanide. We have established a method, which rescues 50% of the CO dehydrogenase activity by in vitro reconstitution of the active site through the supply of sulphide first and subsequently of Cu(I) under reducing conditions. Immature forms of CO dehydrogenase isolated from the bacterium, which were deficient in S and/or Cu at the active site, were similarly activated. X-ray crystallography and electron paramagnetic resonance spectroscopy indicated that the [CuSMoO(2)] cluster was properly reconstructed. However, reconstituted CO dehydrogenase contains mature along with immature forms. The chemical reactions of the reconstitution of CO dehydrogenase are summarized in a model, which assumes resulphuration of the Mo-ion at both equatorial positions at a 1:1 molar ratio. One equatorial Mo-S group reacts with Cu(I) in a productive fashion yielding a mature, functional [CuSMoO(2)] cluster. The other Mo-S group reacts with Cu(I), then Cu(2)S is released and an oxo group is introduced from water, yielding an inactive [MoO(3)] centre.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16091936}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16091936 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16091936}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Molybdenum]] | | [[Category: Molybdenum]] |
| | [[Category: Molybdoprotein]] | | [[Category: Molybdoprotein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:12:07 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:19:32 2008'' |
Revision as of 02:19, 28 July 2008
Template:STRUCTURE 1zxi
Reconstituted CO dehydrogenase from Oligotropha carboxidovorans
Template:ABSTRACT PUBMED 16091936
About this Structure
1ZXI is a Protein complex structure of sequences from Oligotropha carboxidovorans. Full crystallographic information is available from OCA.
Reference
Structural and functional reconstruction in situ of the [CuSMoO2] active site of carbon monoxide dehydrogenase from the carbon monoxide oxidizing eubacterium Oligotropha carboxidovorans., Resch M, Dobbek H, Meyer O, J Biol Inorg Chem. 2005 Aug;10(5):518-28. Epub 2005 Sep 23. PMID:16091936
Page seeded by OCA on Mon Jul 28 05:19:32 2008
