1zy6

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[[Image:1zy6.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1zy6| PDB=1zy6 | SCENE= }}
{{STRUCTURE_1zy6| PDB=1zy6 | SCENE= }}
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'''Membrane-bound dimer structure of Protegrin-1 (PG-1), a beta-Hairpin Antimicrobial Peptide in Lipid Bilayers from Rotational-Echo Double-Resonance Solid-State NMR'''
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===Membrane-bound dimer structure of Protegrin-1 (PG-1), a beta-Hairpin Antimicrobial Peptide in Lipid Bilayers from Rotational-Echo Double-Resonance Solid-State NMR===
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==Overview==
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The intermolecular packing of a beta-hairpin antimicrobial peptide, PG-1, in lipid bilayers is determined using solid-state NMR distance measurements. Previous spin counting experiments showed that PG-1 associates as dimers in POPC bilayers; however, the detailed dimer structure was unknown. We have now measured several intermolecular 13C-19F, 1H-13C, and 15N-13C distances in site-specifically labeled PG-1 to constrain the structure of the intermolecular interface. The distances are measured using the rotational-echo double-resonance (REDOR) technique under magic-angle spinning. The results indicate that two PG-1 molecules align in a parallel fashion with the C-terminal strand of the hairpin forming the dimer interface. Six hydrogen bonds stabilize this interface, and the Phe12 side chain adopts the g- conformation in the membrane as in solution. The parallel packing of the peptide in the lipid bilayer differs from the antiparallel dimer found in DPC micelles and may be stabilized by its strong amphipathic character, which should facilitate its insertion into the amphipathic lipid bilayer. This study demonstrates the utility of the REDOR NMR technique for the elucidation of the oligomeric structure of membrane proteins.
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The line below this paragraph, {{ABSTRACT_PUBMED_16819833}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16819833 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16819833}}
==About this Structure==
==About this Structure==
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1ZY6 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZY6 OCA].
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1ZY6 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZY6 OCA].
==Reference==
==Reference==
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[[Category: Beta-hairpin]]
[[Category: Beta-hairpin]]
[[Category: Solid state nmr]]
[[Category: Solid state nmr]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:13:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:58:05 2008''

Revision as of 02:58, 28 July 2008

Image:1zy6.png

Template:STRUCTURE 1zy6

Membrane-bound dimer structure of Protegrin-1 (PG-1), a beta-Hairpin Antimicrobial Peptide in Lipid Bilayers from Rotational-Echo Double-Resonance Solid-State NMR

Template:ABSTRACT PUBMED 16819833

About this Structure

1ZY6 is a Single protein structure. Full experimental information is available from OCA.

Reference

Membrane-bound dimer structure of a beta-hairpin antimicrobial peptide from rotational-echo double-resonance solid-state NMR., Mani R, Tang M, Wu X, Buffy JJ, Waring AJ, Sherman MA, Hong M, Biochemistry. 2006 Jul 11;45(27):8341-9. PMID:16819833

Page seeded by OCA on Mon Jul 28 05:58:05 2008

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