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256l

From Proteopedia

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[[Image:256l.jpg|left|200px]]
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{{Seed}}
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[[Image:256l.png|left|200px]]
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{{STRUCTURE_256l| PDB=256l | SCENE= }}
{{STRUCTURE_256l| PDB=256l | SCENE= }}
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'''BACTERIOPHAGE T4 LYSOZYME'''
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===BACTERIOPHAGE T4 LYSOZYME===
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==Overview==
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Phage T4 lysozyme consists of two domains between which is formed the active-site cleft of the enzyme. The crystallographically determined thermal displacement parameters for the protein suggested that the amino terminal of the two domains undergoes 'hinge-bending' motion about an axis passing through the waist of the molecule. Such conformational mobility may be important in allowing access of substrates to the active site of the enzyme. We report here a crystallographic study of a mutant T4 lysozyme which demonstrates further the conformational flexibility of the protein. A mutant form of the enzyme with a methionine residue (Met 6) replaced by isoleucine crystallizes with four independent molecules in the crystal lattice. These four molecules have distinctly different conformations. The mutant protein can also crystallize in standard form with a structure very similar to the wild-type protein. Thus the mutant protein can adopt five different crystal conformations. The isoleucine for methionine substitution at the intersection of the two domains of T4 lysozyme apparently enhances the hinge-bending motion presumed to occur in the wild-type protein, without significantly affecting the catalytic activity or thermal stability of the protein.
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The line below this paragraph, {{ABSTRACT_PUBMED_2234094}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 2234094 is the PubMed ID number.
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{{ABSTRACT_PUBMED_2234094}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Lysozyme]]
[[Category: Lysozyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:23:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:11:04 2008''

Revision as of 07:11, 29 July 2008

Image:256l.png

Template:STRUCTURE 256l

BACTERIOPHAGE T4 LYSOZYME

Template:ABSTRACT PUBMED 2234094

About this Structure

256L is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

A mutant T4 lysozyme displays five different crystal conformations., Faber HR, Matthews BW, Nature. 1990 Nov 15;348(6298):263-6. PMID:2234094

Page seeded by OCA on Tue Jul 29 10:11:04 2008

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