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2a6m

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{{STRUCTURE_2a6m| PDB=2a6m | SCENE= }}
{{STRUCTURE_2a6m| PDB=2a6m | SCENE= }}
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'''Crystal Structure of the ISHp608 Transposase'''
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===Crystal Structure of the ISHp608 Transposase===
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==Overview==
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Many bacteria harbor simple transposable elements termed insertion sequences (IS). In Helicobacter pylori, the chimeric IS605 family elements are particularly interesting due to their proximity to genes encoding gastric epithelial invasion factors. Protein sequences of IS605 transposases do not bear the hallmarks of other well-characterized transposases. We have solved the crystal structure of full-length transposase (TnpA) of a representative member, ISHp608. Structurally, TnpA does not resemble any characterized transposase; rather, it is related to rolling circle replication (RCR) proteins. Consistent with RCR, Mg2+ and a conserved tyrosine, Tyr127, are essential for DNA nicking and the formation of a covalent intermediate between TnpA and DNA. TnpA is dimeric, contains two shared active sites, and binds two DNA stem loops representing the conserved inverted repeats near each end of ISHp608. The cocrystal structure with stem-loop DNA illustrates how this family of transposases specifically recognizes and pairs ends, necessary steps during transposition.
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(as it appears on PubMed at http://www.pubmed.gov), where 16209952 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16209952}}
==About this Structure==
==About this Structure==
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[[Category: Ton-Hoang, B.]]
[[Category: Ton-Hoang, B.]]
[[Category: Rna recognition motif]]
[[Category: Rna recognition motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:40:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:39:25 2008''

Revision as of 09:39, 28 July 2008

Image:2a6m.png

Template:STRUCTURE 2a6m

Crystal Structure of the ISHp608 Transposase

Template:ABSTRACT PUBMED 16209952

About this Structure

2A6M is a Single protein structure of sequence from Helicobacter pylori. Full crystallographic information is available from OCA.

Reference

Active site sharing and subterminal hairpin recognition in a new class of DNA transposases., Ronning DR, Guynet C, Ton-Hoang B, Perez ZN, Ghirlando R, Chandler M, Dyda F, Mol Cell. 2005 Oct 7;20(1):143-54. PMID:16209952

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