2a8k

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2a8k.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2a8k.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2a8k| PDB=2a8k | SCENE= }}
{{STRUCTURE_2a8k| PDB=2a8k | SCENE= }}
-
'''Structural and Mutational Studies of the Catalytic Domain of Colicin E5a tRNA-Specific Ribonuclease'''
+
===Structural and Mutational Studies of the Catalytic Domain of Colicin E5a tRNA-Specific Ribonuclease===
-
==Overview==
+
<!--
-
Colicin E5 specifically cleaves four tRNAs in Escherichia coli that contain the modified nucleotide queuosine (Q) at the wobble position, thereby preventing protein synthesis and ultimately resulting in cell death. Here, the crystal structure of the catalytic domain of colicin E5 (E5-CRD) from E. coli was determined at 1.5 A resolution. Unexpectedly, E5-CRD adopts a core folding with a four-stranded beta-sheet packed against an alpha-helix, seen in the well-studied ribonuclease T1 despite a lack of sequence similarity. Beyond the core catalytic domain, an N-terminal helix, a C-terminal beta-strand and loop, and an extended internal loop constitute an RNA binding cleft. Mutational analysis identified five amino acids that were important for tRNA substrate binding and cleavage by E5-CRD. The structure, together with the mutational study, allows us to propose a model of colicin E5-tRNA interactions, suggesting the molecular basis of tRNA substrate recognition and the mechanism of tRNA cleavage by colicin E5.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16060658}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16060658 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16060658}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Ribonuclease]]
[[Category: Ribonuclease]]
[[Category: Toxin]]
[[Category: Toxin]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:44:51 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:52:50 2008''

Revision as of 11:52, 28 July 2008

Image:2a8k.png

Template:STRUCTURE 2a8k

Structural and Mutational Studies of the Catalytic Domain of Colicin E5a tRNA-Specific Ribonuclease

Template:ABSTRACT PUBMED 16060658

About this Structure

2A8K is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural and mutational studies of the catalytic domain of colicin E5: a tRNA-specific ribonuclease., Lin YL, Elias Y, Huang RH, Biochemistry. 2005 Aug 9;44(31):10494-500. PMID:16060658

Page seeded by OCA on Mon Jul 28 14:52:50 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2a8k"
Personal tools