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2aak

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{{STRUCTURE_2aak| PDB=2aak | SCENE= }}
{{STRUCTURE_2aak| PDB=2aak | SCENE= }}
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'''UBIQUITIN CONJUGATING ENZYME FROM ARABIDOPSIS THALIANA'''
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===UBIQUITIN CONJUGATING ENZYME FROM ARABIDOPSIS THALIANA===
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==Overview==
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The x-ray crystal structure of a recombinant ubiquitin-conjugating enzyme (E2) encoded by the UBC1 gene of the plant Arabidopsis thaliana has been determined with the use of multiple isomorphous replacement techniques and refined at 2.4-A resolution by simulated annealing and restrained least-squares. This E2 is an alpha/beta protein, with four alpha-helices and a four-stranded antiparallel beta-sheet. The NH2 and COOH termini, which may be important for interaction with other enzymes and substrates in the ubiquitin-conjugation pathway, are on the opposite side of the molecule from the cysteine residue that binds to the COOH terminus of ubiquitin. This structure should now allow for the rational analysis of E2 function by in vitro mutagenesis and facilitate the effective design of E2s with unique specificities or catalytic functions.
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(as it appears on PubMed at http://www.pubmed.gov), where 1321826 is the PubMed ID number.
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{{ABSTRACT_PUBMED_1321826}}
==About this Structure==
==About this Structure==
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[[Category: Ligase]]
[[Category: Ligase]]
[[Category: Ubiquitin conjugation]]
[[Category: Ubiquitin conjugation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:49:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:21:29 2008''

Revision as of 06:21, 29 July 2008

Image:2aak.png

Template:STRUCTURE 2aak

UBIQUITIN CONJUGATING ENZYME FROM ARABIDOPSIS THALIANA

Template:ABSTRACT PUBMED 1321826

About this Structure

2AAK is a Single protein structure of sequence from Arabidopsis thaliana. This structure supersedes the now removed PDB entry 1aak. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of a ubiquitin-conjugating enzyme (E2)., Cook WJ, Jeffrey LC, Sullivan ML, Vierstra RD, J Biol Chem. 1992 Jul 25;267(21):15116-21. PMID:1321826

Page seeded by OCA on Tue Jul 29 09:21:29 2008

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