2abk

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{{STRUCTURE_2abk| PDB=2abk | SCENE= }}
{{STRUCTURE_2abk| PDB=2abk | SCENE= }}
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'''REFINEMENT OF THE NATIVE STRUCTURE OF ENDONUCLEASE III TO A RESOLUTION OF 1.85 ANGSTROM'''
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===REFINEMENT OF THE NATIVE STRUCTURE OF ENDONUCLEASE III TO A RESOLUTION OF 1.85 ANGSTROM===
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==Overview==
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The 1.85 A crystal structure of endonuclease III, combined with mutational analysis, suggests the structural basis for the DNA binding and catalytic activity of the enzyme. Helix-hairpin-helix (HhH) and [4Fe-4S] cluster loop (FCL) motifs, which we have named for their secondary structure, bracket the cleft separating the two alpha-helical domains of the enzyme. These two novel DNA binding motifs and the solvent-filled pocket in the cleft between them all lie within a positively charged and sequence-conserved surface region. Lys120 and Asp138, both shown by mutagenesis to be catalytically important, lie at the mouth of this pocket, suggesting that this pocket is part of the active site. The positions of the HhH motif and protruding FCL motif, which contains the DNA binding residue Lys191, can accommodate B-form DNA, with a flipped-out base bound within the active site pocket. The identification of HhH and FCL sequence patterns in other DNA binding proteins suggests that these motifs may be a recurrent structural theme for DNA binding proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 7664751 is the PubMed ID number.
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{{ABSTRACT_PUBMED_7664751}}
==About this Structure==
==About this Structure==
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[[Category: Dna glycosylase]]
[[Category: Dna glycosylase]]
[[Category: Dna-repair]]
[[Category: Dna-repair]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:51:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:55:28 2008''

Revision as of 12:55, 28 July 2008

Image:2abk.png

Template:STRUCTURE 2abk

REFINEMENT OF THE NATIVE STRUCTURE OF ENDONUCLEASE III TO A RESOLUTION OF 1.85 ANGSTROM

Template:ABSTRACT PUBMED 7664751

About this Structure

2ABK is a Single protein structure of sequence from Escherichia coli. This structure supersedes the now removed PDB entry 1abk. Full crystallographic information is available from OCA.

Reference

Novel DNA binding motifs in the DNA repair enzyme endonuclease III crystal structure., Thayer MM, Ahern H, Xing D, Cunningham RP, Tainer JA, EMBO J. 1995 Aug 15;14(16):4108-20. PMID:7664751

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