2abz

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[[Image:2abz.gif|left|200px]]
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{{STRUCTURE_2abz| PDB=2abz | SCENE= }}
{{STRUCTURE_2abz| PDB=2abz | SCENE= }}
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'''Crystal structure of C19A/C43A mutant of leech carboxypeptidase inhibitor in complex with bovine carboxypeptidase A'''
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===Crystal structure of C19A/C43A mutant of leech carboxypeptidase inhibitor in complex with bovine carboxypeptidase A===
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==Overview==
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The oxidative folding pathway of leech carboxypeptidase inhibitor (LCI; four disulfide bonds) proceeds through the formation of two major intermediates (III-A and III-B) that contain three native disulfide bonds and act as strong kinetic traps in the folding process. The III-B intermediate lacks the Cys19-Cys43 disulfide bond that links the beta-sheet core with the alpha-helix in wild-type LCI. Here, an analog of this intermediate was constructed by replacing Cys19 and Cys43 with alanine residues. Its oxidative folding follows a rapid sequential flow through one, two, and three disulfide species to reach the native form; the low accumulation of two disulfide intermediates and three disulfide (scrambled) isomers accounts for a highly efficient reaction. The three-dimensional structure of this analog, alone and in complex with carboxypeptidase A (CPA), was determined by X-ray crystallography at 2.2A resolution. Its overall structure is very similar to that of wild-type LCI, although the residues in the region adjacent to the mutation sites show an increased flexibility, which is strongly reduced upon binding to CPA. The structure of the complex also demonstrates that the analog and the wild-type LCI bind to the enzyme in the same manner, as expected by their inhibitory capabilities, which were similar for all enzymes tested. Equilibrium unfolding experiments showed that this mutant is destabilized by approximately 1.5 kcal mol(-1) (40%) relative to the wild-type protein. Together, the data indicate that the fourth disulfide bond provides LCI with both high stability and structural specificity.
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(as it appears on PubMed at http://www.pubmed.gov), where 16126224 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16126224}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Study of a major intermediate in the oxidative folding of leech carboxypeptidase inhibitor: contribution of the fourth disulfide bond., Arolas JL, Popowicz GM, Bronsoms S, Aviles FX, Huber R, Holak TA, Ventura S, J Mol Biol. 2005 Sep 30;352(4):961-75. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16126224 16126224]
Study of a major intermediate in the oxidative folding of leech carboxypeptidase inhibitor: contribution of the fourth disulfide bond., Arolas JL, Popowicz GM, Bronsoms S, Aviles FX, Huber R, Holak TA, Ventura S, J Mol Biol. 2005 Sep 30;352(4):961-75. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16126224 16126224]
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Structure of a novel leech carboxypeptidase inhibitor determined free in solution and in complex with human carboxypeptidase A2., Reverter D, Fernandez-Catalan C, Baumgartner R, Pfander R, Huber R, Bode W, Vendrell J, Holak TA, Aviles FX, Nat Struct Biol. 2000 Apr;7(4):322-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10742178 10742178]
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NMR structural characterization and computational predictions of the major intermediate in oxidative folding of leech carboxypeptidase inhibitor., Arolas JL, D'Silva L, Popowicz GM, Aviles FX, Holak TA, Ventura S, Structure. 2005 Aug;13(8):1193-202. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16084391 16084391]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Carboxypeptidase A]]
[[Category: Carboxypeptidase A]]
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[[Category: Lci mutant]]
[[Category: Lci mutant]]
[[Category: Oxidative folding intermediate analog]]
[[Category: Oxidative folding intermediate analog]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:51:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:10:01 2008''

Revision as of 03:10, 28 July 2008

Image:2abz.png

Template:STRUCTURE 2abz

Crystal structure of C19A/C43A mutant of leech carboxypeptidase inhibitor in complex with bovine carboxypeptidase A

Template:ABSTRACT PUBMED 16126224

About this Structure

2ABZ is a Protein complex structure of sequences from Bos taurus and Hirudo medicinalis. Full crystallographic information is available from OCA.

Reference

Study of a major intermediate in the oxidative folding of leech carboxypeptidase inhibitor: contribution of the fourth disulfide bond., Arolas JL, Popowicz GM, Bronsoms S, Aviles FX, Huber R, Holak TA, Ventura S, J Mol Biol. 2005 Sep 30;352(4):961-75. PMID:16126224

Structure of a novel leech carboxypeptidase inhibitor determined free in solution and in complex with human carboxypeptidase A2., Reverter D, Fernandez-Catalan C, Baumgartner R, Pfander R, Huber R, Bode W, Vendrell J, Holak TA, Aviles FX, Nat Struct Biol. 2000 Apr;7(4):322-8. PMID:10742178

NMR structural characterization and computational predictions of the major intermediate in oxidative folding of leech carboxypeptidase inhibitor., Arolas JL, D'Silva L, Popowicz GM, Aviles FX, Holak TA, Ventura S, Structure. 2005 Aug;13(8):1193-202. PMID:16084391

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