2acw

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{{STRUCTURE_2acw| PDB=2acw | SCENE= }}
{{STRUCTURE_2acw| PDB=2acw | SCENE= }}
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'''Crystal Structure of Medicago truncatula UGT71G1 complexed with UDP-glucose'''
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===Crystal Structure of Medicago truncatula UGT71G1 complexed with UDP-glucose===
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==Overview==
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Glycosylation is a ubiquitous reaction controlling the bioactivity and storage of plant natural products. Glycosylation of small molecules is catalyzed by a superfamily of glycosyltransferases (GTs) in most plant species studied to date. We present crystal structures of the UDP flavonoid/triterpene GT UGT71G1 from Medicago truncatula bound to UDP or UDP-glucose. The structures reveal the key residues involved in the recognition of donor substrate and, by comparison with other GT structures, suggest His-22 as the catalytic base and Asp-121 as a key residue that may assist deprotonation of the acceptor by forming an electron transfer chain with the catalytic base. Mutagenesis confirmed the roles of these key residues in donor substrate binding and enzyme activity. Our results provide an initial structural basis for understanding the complex substrate specificity and regiospecificity underlying the glycosylation of plant natural products and other small molecules. This information will direct future attempts to engineer bioactive compounds in crop plants to improve plant, animal, and human health and to facilitate the rational design of GTs to improve the storage and stability of novel engineered bioactive compounds.
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(as it appears on PubMed at http://www.pubmed.gov), where 16214900 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16214900}}
==About this Structure==
==About this Structure==
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[[Category: Glycosyltransferase]]
[[Category: Glycosyltransferase]]
[[Category: Udp-glucose]]
[[Category: Udp-glucose]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:53:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:34:17 2008''

Revision as of 18:34, 28 July 2008

Image:2acw.png

Template:STRUCTURE 2acw

Crystal Structure of Medicago truncatula UGT71G1 complexed with UDP-glucose

Template:ABSTRACT PUBMED 16214900

About this Structure

2ACW is a Single protein structure of sequence from Medicago truncatula. Full crystallographic information is available from OCA.

Reference

Crystal structures of a multifunctional triterpene/flavonoid glycosyltransferase from Medicago truncatula., Shao H, He X, Achnine L, Blount JW, Dixon RA, Wang X, Plant Cell. 2005 Nov;17(11):3141-54. Epub 2005 Oct 7. PMID:16214900

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