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2afq

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[[Image:2afq.gif|left|200px]]
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{{STRUCTURE_2afq| PDB=2afq | SCENE= }}
{{STRUCTURE_2afq| PDB=2afq | SCENE= }}
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'''1.9 angstrom crytal structure of wild-type human thrombin in the sodium free state'''
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===1.9 angstrom crytal structure of wild-type human thrombin in the sodium free state===
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==Overview==
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Regulation of thrombin activity is critical for haemostasis and the prevention of thrombosis. Thrombin has several procoagulant substrates, including fibrinogen and platelet receptors, and essential cofactors for stimulating its own formation. However, thrombin is also capable of serving an anticoagulant function by activating protein C. The specificity of thrombin is primarily regulated by binding to the cofactor TM (thrombomodulin), but co-ordination of Na+ can also affect thrombin activity. The Na+-free form is often referred to as 'slow' because of reduced rates of cleavage of procoagulant substrates, but the slow form is still capable of rapid activation of protein C in the presence of TM. The molecular basis of the slow proteolytic activity of thrombin has remained elusive, in spite of two decades of solution studies and many published crystallographic structures. In the present paper, we report the first structure of wild-type unliganded human thrombin grown in the absence of co-ordinating Na+. The Na+-binding site is observed in a highly ordered position 6 A (1 A=0.1 nm) removed from that seen in the Na+-bound state. The movement of the Na+ loop results in non-catalytic hydrogen-bonding in the active site and blocking of the S1 and S2 substrate-binding pockets. Similar, if more dramatic, changes were observed in a previous structure of the constitutively slow thrombin variant E217K. The slow behaviour of thrombin in solutions devoid of Na+ can now be understood in terms of an equilibrium between an inert species, represented by the crystal structure described in the present paper, and an active form, where the addition of Na+ populates the active state.
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(as it appears on PubMed at http://www.pubmed.gov), where 16201969 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16201969}}
==Disease==
==Disease==
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[[Category: Coagulation factor ii]]
[[Category: Coagulation factor ii]]
[[Category: Protease]]
[[Category: Protease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:59:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:28:56 2008''

Revision as of 00:29, 29 July 2008

Image:2afq.png

Template:STRUCTURE 2afq

Contents

1.9 angstrom crytal structure of wild-type human thrombin in the sodium free state

Template:ABSTRACT PUBMED 16201969

Disease

Known disease associated with this structure: Dysprothrombinemia OMIM:[176930], Hyperprothrombinemia OMIM:[176930], Hypoprothrombinemia OMIM:[176930]

About this Structure

2AFQ is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of wild-type human thrombin in the Na+-free state., Johnson DJ, Adams TE, Li W, Huntington JA, Biochem J. 2005 Nov 15;392(Pt 1):21-8. PMID:16201969

Page seeded by OCA on Tue Jul 29 03:28:56 2008

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