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2ag6

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{{STRUCTURE_2ag6| PDB=2ag6 | SCENE= }}
{{STRUCTURE_2ag6| PDB=2ag6 | SCENE= }}
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'''Crystal structure of p-bromo-l-phenylalanine-tRNA sythetase in complex with p-bromo-l-phenylalanine'''
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===Crystal structure of p-bromo-l-phenylalanine-tRNA sythetase in complex with p-bromo-l-phenylalanine===
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==Overview==
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Recently, tRNA aminoacyl-tRNA synthetase pairs have been evolved that allow one to genetically encode a large array of unnatural amino acids in both prokaryotic and eukaryotic organisms. We have determined the crystal structures of two substrate-bound Methanococcus jannaschii tyrosyl aminoacyl-tRNA synthetases that charge the unnatural amino acids p-bromophenylalanine and 3-(2-naphthyl)alanine (NpAla). A comparison of these structures with the substrate-bound WT synthetase, as well as a mutant synthetase that charges p-acetylphenylalanine, shows that altered specificity is due to both side-chain and backbone rearrangements within the active site that modify hydrogen bonds and packing interactions with substrate, as well as disrupt the alpha8-helix, which spans the WT active site. The high degree of structural plasticity that is observed in these aminoacyl-tRNA synthetases is rarely found in other mutant enzymes with altered specificities and provides an explanation for the surprising adaptability of the genetic code to novel amino acids.
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(as it appears on PubMed at http://www.pubmed.gov), where 16618920 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16618920}}
==About this Structure==
==About this Structure==
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[[Category: Trna synthetase]]
[[Category: Trna synthetase]]
[[Category: Unnatural amino acid]]
[[Category: Unnatural amino acid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:00:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:29:01 2008''

Revision as of 04:29, 28 July 2008

Image:2ag6.png

Template:STRUCTURE 2ag6

Crystal structure of p-bromo-l-phenylalanine-tRNA sythetase in complex with p-bromo-l-phenylalanine

Template:ABSTRACT PUBMED 16618920

About this Structure

2AG6 is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

Reference

Structural plasticity of an aminoacyl-tRNA synthetase active site., Turner JM, Graziano J, Spraggon G, Schultz PG, Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6483-8. Epub 2006 Apr 17. PMID:16618920

Page seeded by OCA on Mon Jul 28 07:29:01 2008

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