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2ahc

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{{STRUCTURE_2ahc| PDB=2ahc | SCENE= }}
{{STRUCTURE_2ahc| PDB=2ahc | SCENE= }}
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'''Chorismate lyase with inhibitor Vanilate'''
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===Chorismate lyase with inhibitor Vanilate===
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==Overview==
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Chorismate lyase (CL) removes the pyruvyl group from chorismate to provide 4-hydroxybenzoate (4HB) for the ubiquinone pathway. We previously reported the crystal structure at 1.4A resolution of the Escherichia coli CL with bound 4HB product, showing that the product is bound in an internal cavity behind two flaps. To provide a more complete basis for understanding CL's unusual ligand-binding properties and mechanism of action, we now report four crystal structures of CL mutants and inhibitor complexes, together with binding and activity measurements and molecular dynamics simulations. First, an ultrahigh resolution (1.0A) crystal structure of the CL*product complex reveals details of a substrate-sized internal cavity, also behind the flaps, near the product site. Second, a 2.4A structure of CL complexed with the inhibitor vanillate shows the flaps partly opened relative to their product-bound positions. Third, a 2.0A structure of the G90A mutant with bound product reveals the basis for tighter product binding and kinetic effects of this active site mutation. Fourth, the combination of the G90A mutation with the vanillate inhibitor produces a 1.9A structure containing two inhibitor molecules, one in the product site and the other in the adjacent cavity. The two sites are connected by a short tunnel that is partly open at each end, suggesting that CL may operate via a 2-site or tunnel mechanism.
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{{ABSTRACT_PUBMED_16343413}}
==About this Structure==
==About this Structure==
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[[Category: Ubiquinone pathway]]
[[Category: Ubiquinone pathway]]
[[Category: Unique fold]]
[[Category: Unique fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:02:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:11:01 2008''

Revision as of 17:11, 27 July 2008

Image:2ahc.png

Template:STRUCTURE 2ahc

Chorismate lyase with inhibitor Vanilate

Template:ABSTRACT PUBMED 16343413

About this Structure

2AHC is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural analysis of ligand binding and catalysis in chorismate lyase., Smith N, Roitberg AE, Rivera E, Howard A, Holden MJ, Mayhew M, Kaistha S, Gallagher DT, Arch Biochem Biophys. 2006 Jan 1;445(1):72-80. Epub 2005 Nov 22. PMID:16343413

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