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2ahq

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[[Image:2ahq.gif|left|200px]]
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{{STRUCTURE_2ahq| PDB=2ahq | SCENE= }}
{{STRUCTURE_2ahq| PDB=2ahq | SCENE= }}
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'''Solution Structure of the C-terminal RpoN Domain of Sigma-54 from Aquifex aeolicus'''
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===Solution Structure of the C-terminal RpoN Domain of Sigma-54 from Aquifex aeolicus===
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==Overview==
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The "sigma" subunit of prokaryotic RNA polymerase allows gene-specific transcription initiation. Two sigma families have been identified, sigma70 and sigma54, which use distinct mechanisms to initiate transcription and share no detectable sequence homology. Although the sigma70-type factors have been well characterized structurally by x-ray crystallography, no high resolution structural information is available for the sigma54-type factors. Here we present the NMR-derived structure of the C-terminal domain of sigma54 from Aquifex aeolicus. This domain (Thr-323 to Gly-389), which contains the highly conserved RpoN box sequence, consists of a poorly structured N-terminal tail followed by a three-helix bundle, which is surprisingly similar to domains of the sigma70-type proteins. Residues of the RpoN box, which have previously been shown to be critical for DNA binding, form the second helix of an unpredicted helix-turn-helix motif. The homology of this structure with other DNA-binding proteins, combined with previous biochemical data, suggests how the C-terminal domain of sigma54 binds to DNA.
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(as it appears on PubMed at http://www.pubmed.gov), where 16210314 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16210314}}
==About this Structure==
==About this Structure==
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2AHQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHQ OCA].
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2AHQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHQ OCA].
==Reference==
==Reference==
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[[Category: Solution structure]]
[[Category: Solution structure]]
[[Category: Transcription]]
[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:03:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 18:41:47 2008''

Revision as of 15:41, 28 July 2008

Image:2ahq.png

Template:STRUCTURE 2ahq

Solution Structure of the C-terminal RpoN Domain of Sigma-54 from Aquifex aeolicus

Template:ABSTRACT PUBMED 16210314

About this Structure

2AHQ is a Single protein structure of sequence from Aquifex aeolicus. Full experimental information is available from OCA.

Reference

The C-terminal RpoN domain of sigma54 forms an unpredicted helix-turn-helix motif similar to domains of sigma70., Doucleff M, Malak LT, Pelton JG, Wemmer DE, J Biol Chem. 2005 Dec 16;280(50):41530-6. Epub 2005 Oct 6. PMID:16210314

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