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| - | [[Image:2ai5.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2ai5| PDB=2ai5 | SCENE= }} | | {{STRUCTURE_2ai5| PDB=2ai5 | SCENE= }} |
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| - | '''Solution Structure of Cytochrome C552, determined by Distributed Computing Implementation for NMR data'''
| + | ===Solution Structure of Cytochrome C552, determined by Distributed Computing Implementation for NMR data=== |
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| - | ==Overview==
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| - | We have studied the structure-thermostability relationship using cytochromes c from mesophilic and thermophilic bacteria; Pseudomonas aeruginosa (PAc(551)) growing at 37 degrees C and Hydrogenobacter thermophilus (HTc(552)) at 72 degrees C and showed that only five residues primarily differentiate their stabilities. For a more comprehensive study, we found Hydrogenophilus thermoluteolus (Pseudomonas hydrogenothermophila) growing at 52 degrees C and showed the moderate stability of the cytochrome c from this bacterium (PHc(552)). To explore the stabilization mechanisms, the crystal structure of PHc(552) was determined by X-ray analysis. The solution structure of HTc(552) elucidated previously by NMR was refined using distributed computational implementation. Furthermore, the recently reported crystal structure of HTc(552) has become available [Travaglini-Allocatelli, C. et al. (2005) J. Biol. Chem. 280, 25729-25734]. When the structures of these three cytochromes c were combined, this revealed that the five residues, corresponding to those mentioned above, determine the difference of stabilities among them as well. These facts suggested the stabilization mechanisms as follows: (1) improved van der Waals interactions by packing optimization at the N-terminal helix, (2) attractive electrostatic interactions with the heme propionate group, and (3) favorable van der Waals interaction with the heme. This comparative study, by supplementing the structural information of PHc(552) with its complementary feature, demonstrates that just a small number of amino acid residues determine the overall molecular stability by means of additivity of the effects of their substitutions. It is interesting that, in naturally occurring proteins, these adaptation strategies are accommodated by these bacteria to survive in the wide range of thermal conditions.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16681384}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16681384 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16681384}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 2AI5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hydrogenobacter_thermophilus Hydrogenobacter thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AI5 OCA]. | + | 2AI5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hydrogenobacter_thermophilus Hydrogenobacter thermophilus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AI5 OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Ferrous iron]] | | [[Category: Ferrous iron]] |
| | [[Category: Porphyrin]] | | [[Category: Porphyrin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:04:47 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:45:37 2008'' |
Revision as of 11:45, 27 July 2008
Template:STRUCTURE 2ai5
Solution Structure of Cytochrome C552, determined by Distributed Computing Implementation for NMR data
Template:ABSTRACT PUBMED 16681384
About this Structure
2AI5 is a Single protein structure of sequence from Hydrogenobacter thermophilus. Full experimental information is available from OCA.
Reference
Structure of cytochrome c552 from a moderate thermophilic bacterium, Hydrogenophilus thermoluteolus: comparative study on the thermostability of cytochrome c., Nakamura S, Ichiki S, Takashima H, Uchiyama S, Hasegawa J, Kobayashi Y, Sambongi Y, Ohkubo T, Biochemistry. 2006 May 16;45(19):6115-23. PMID:16681384
Page seeded by OCA on Sun Jul 27 14:45:37 2008
