2aje

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2aje.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2aje.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2aje| PDB=2aje | SCENE= }}
{{STRUCTURE_2aje| PDB=2aje | SCENE= }}
-
'''Solution structure of the Arabidopsis thaliana telomeric repeat-binding protein DNA binding domain'''
+
===Solution structure of the Arabidopsis thaliana telomeric repeat-binding protein DNA binding domain===
-
==Overview==
+
<!--
-
The double-stranded telomeric repeat-binding protein (TRP) AtTRP1 is isolated from Arabidopsis thaliana. Using gel retardation assays, we defined the C-terminal 97 amino acid residues, Gln464 to Val560 (AtTRP1(464-560)), as the minimal structured telomeric repeat-binding domain. This region contains a typical Myb DNA-binding motif and a C-terminal extension of 40 amino acid residues. The monomeric AtTRP1(464-560) binds to a 13-mer DNA duplex containing a single repeat of an A.thaliana telomeric DNA sequence (GGTTTAG) in a 1:1 complex, with a K(D) approximately 10(-6)-10(-7) M. Nuclear magnetic resonance (NMR) examination revealed that the solution structure of AtTRP1(464-560) is a novel four-helix tetrahedron rather than the three-helix bundle structure found in typical Myb motifs and other TRPs. Binding of the 13-mer DNA duplex to AtTRP1(464-560) induced significant chemical shift perturbations of protein amide resonances, which suggests that helix 3 (H3) and the flexible loop connecting H3 and H4 are essential for telomeric DNA sequence recognition. Furthermore, similar to that in hTRF1, the N-terminal arm likely contributes to or stabilizes DNA binding. Sequence comparisons suggested that the four-helix structure and the involvement of the loop residues in DNA binding may be features unique to plant TRPs.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16337232}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16337232 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16337232}}
==About this Structure==
==About this Structure==
-
2AJE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJE OCA].
+
2AJE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AJE OCA].
==Reference==
==Reference==
Line 31: Line 35:
[[Category: Telomere]]
[[Category: Telomere]]
[[Category: Trp]]
[[Category: Trp]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:07:27 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:43:33 2008''

Revision as of 04:43, 28 July 2008

Image:2aje.png

Template:STRUCTURE 2aje

Solution structure of the Arabidopsis thaliana telomeric repeat-binding protein DNA binding domain

Template:ABSTRACT PUBMED 16337232

About this Structure

2AJE is a Single protein structure of sequence from Arabidopsis thaliana. Full experimental information is available from OCA.

Reference

Solution structure of the Arabidopsis thaliana telomeric repeat-binding protein DNA binding domain: a new fold with an additional C-terminal helix., Sue SC, Hsiao HH, Chung BC, Cheng YH, Hsueh KL, Chen CM, Ho CH, Huang TH, J Mol Biol. 2006 Feb 10;356(1):72-85. Epub 2005 Nov 22. PMID:16337232

Page seeded by OCA on Mon Jul 28 07:43:33 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2aje"
Personal tools