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2ap0

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[[Image:2ap0.gif|left|200px]]
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{{STRUCTURE_2ap0| PDB=2ap0 | SCENE= }}
{{STRUCTURE_2ap0| PDB=2ap0 | SCENE= }}
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'''Solution Structure of the C27A ScYLV P1-P2 Frameshifting Pseudoknot, 20 Lowest Energy Structures'''
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===Solution Structure of the C27A ScYLV P1-P2 Frameshifting Pseudoknot, 20 Lowest Energy Structures===
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==Overview==
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The helical junction region of a -1 frameshift stimulating hairpin-type mRNA pseudoknot from sugarcane yellow leaf virus (ScYLV) is characterized by a novel C27.(G7-C14) loop 2-stem 1 minor groove base triple, which is stacked on a C8+.(G12-C28) loop 1-stem 2 major groove base triple. Substitution of C27 with adenosine reduces frameshifting efficiency to a level just twofold above the slip-site alone. Here, we show that the global structure of the C27A ScYLV RNA is nearly indistinguishable from the wild-type counterpart, despite the fact that the helical junction region is altered and incorporates the anticipated isostructural A27.(G7-C14) minor groove base triple. This interaction mediates a 2.3-A displacement of C8+ driven by an A27 N6-C8+ O2 hydrogen bond as part of an A(n-1).C+.G-Cn base quadruple. The helical junction regions of the C27A ScYLV and the beet western yellows virus (BWYV) pseudoknots are essentially superimposable, the latter of which contains an analogous A25.(G7-C14) minor groove base triple. These results reveal that the global ground-state structure is not strongly correlated with frameshift stimulation and point to a reduced thermodynamic stability and/or enhanced kinetic lability that derives from an altered helical junction architecture in the C27A ScYLV RNA as a significant determinant for setting frameshifting efficiencies in plant luteoviral mRNA pseudoknots.
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The line below this paragraph, {{ABSTRACT_PUBMED_17000902}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17000902 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17000902}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AP0 OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AP0 OCA].
==Reference==
==Reference==
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[[Category: Frameshifting]]
[[Category: Frameshifting]]
[[Category: Rna pseudoknot]]
[[Category: Rna pseudoknot]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:18:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:34:37 2008''

Revision as of 05:34, 28 July 2008

Image:2ap0.png

Template:STRUCTURE 2ap0

Solution Structure of the C27A ScYLV P1-P2 Frameshifting Pseudoknot, 20 Lowest Energy Structures

Template:ABSTRACT PUBMED 17000902

About this Structure

Full experimental information is available from OCA.

Reference

The global structures of a wild-type and poorly functional plant luteoviral mRNA pseudoknot are essentially identical., Cornish PV, Stammler SN, Giedroc DP, RNA. 2006 Nov;12(11):1959-69. Epub 2006 Sep 25. PMID:17000902

Page seeded by OCA on Mon Jul 28 08:34:37 2008

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