2ar8

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[[Image:2ar8.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_2ar8| PDB=2ar8 | SCENE= }}
{{STRUCTURE_2ar8| PDB=2ar8 | SCENE= }}
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'''The structure of tryptophan 7-halogenase (PrnA)suggests a mechanism for regioselective chlorination'''
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===The structure of tryptophan 7-halogenase (PrnA)suggests a mechanism for regioselective chlorination===
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==Overview==
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Chlorinated natural products include vancomycin and cryptophycin A. Their biosynthesis involves regioselective chlorination by flavin-dependent halogenases. We report the structural characterization of tryptophan 7-halogenase (PrnA), which regioselectively chlorinates tryptophan. Tryptophan and flavin adenine dinucleotide (FAD) are separated by a 10 angstrom-long tunnel and bound by distinct enzyme modules. The FAD module is conserved in halogenases and is related to flavin-dependent monooxygenases. On the basis of biochemical studies, crystal structures, and by analogy with monooxygenases, we predict that FADH2 reacts with O2 to make peroxyflavin, which is decomposed by Cl-. The resulting HOCl is guided through the tunnel to tryptophan, where it is activated to participate in electrophilic aromatic substitution.
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The line below this paragraph, {{ABSTRACT_PUBMED_16195462}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16195462 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16195462}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination., Dong C, Flecks S, Unversucht S, Haupt C, van Pee KH, Naismith JH, Science. 2005 Sep 30;309(5744):2216-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16195462 16195462]
Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination., Dong C, Flecks S, Unversucht S, Haupt C, van Pee KH, Naismith JH, Science. 2005 Sep 30;309(5744):2216-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16195462 16195462]
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Crystallization and X-ray diffraction of a halogenating enzyme, tryptophan 7-halogenase, from Pseudomonas fluorescens., Dong C, Kotzsch A, Dorward M, van Pee KH, Naismith JH, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1438-40. Epub 2004, Jul 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15272170 15272170]
[[Category: Pseudomonas fluorescens]]
[[Category: Pseudomonas fluorescens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Tryptophan 7-halogenase,flavin-dependent halogenase]]
[[Category: Tryptophan 7-halogenase,flavin-dependent halogenase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:22:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:39:05 2008''

Revision as of 02:39, 29 July 2008

Image:2ar8.png

Template:STRUCTURE 2ar8

The structure of tryptophan 7-halogenase (PrnA)suggests a mechanism for regioselective chlorination

Template:ABSTRACT PUBMED 16195462

About this Structure

2AR8 is a Single protein structure of sequence from Pseudomonas fluorescens. Full crystallographic information is available from OCA.

Reference

Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination., Dong C, Flecks S, Unversucht S, Haupt C, van Pee KH, Naismith JH, Science. 2005 Sep 30;309(5744):2216-9. PMID:16195462

Crystallization and X-ray diffraction of a halogenating enzyme, tryptophan 7-halogenase, from Pseudomonas fluorescens., Dong C, Kotzsch A, Dorward M, van Pee KH, Naismith JH, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1438-40. Epub 2004, Jul 21. PMID:15272170

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