We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

2ar9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2ar9.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2ar9.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2ar9| PDB=2ar9 | SCENE= }}
{{STRUCTURE_2ar9| PDB=2ar9 | SCENE= }}
-
'''Crystal structure of a dimeric caspase-9'''
+
===Crystal structure of a dimeric caspase-9===
-
==Overview==
+
<!--
-
Caspases are responsible for the execution of programmed cell death (apoptosis) and must undergo proteolytic activation, in response to apoptotic stimuli, to function. The mechanism of initiator caspase activation has been generalized by the induced proximity model, which is thought to drive dimerization-mediated activation of caspases. The initiator caspase, caspase-9, exists predominantly as a monomer in solution. To examine the induced proximity model, we engineered a constitutively dimeric caspase-9 by relieving steric hindrance at the dimer interface. Crystal structure of the engineered caspase-9 closely resembles that of the wild-type (WT) caspase-9, including all relevant structural details and the asymmetric nature of two monomers. Compared to the WT caspase-9, this engineered dimer exhibits a higher level of catalytic activity in vitro and induces more efficient cell death when expressed. However, the catalytic activity of the dimeric caspase-9 is only a small fraction of that for the Apaf-1-activated caspase-9. Furthermore, in contrast to the WT caspase-9, the activity of the dimeric caspase-9 can no longer be significantly enhanced in an Apaf-1-dependent manner. These findings suggest that dimerization of caspase-9 may be qualitatively different from its activation by Apaf-1, and in conjunction with other evidence, posit an induced conformation model for the activation of initiator caspases.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15941357}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15941357 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15941357}}
==About this Structure==
==About this Structure==
Line 33: Line 37:
[[Category: Engineered caspase-9]]
[[Category: Engineered caspase-9]]
[[Category: Initiator caspase]]
[[Category: Initiator caspase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:22:37 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:22:23 2008''

Revision as of 13:22, 29 July 2008

Image:2ar9.png

Template:STRUCTURE 2ar9

Crystal structure of a dimeric caspase-9

Template:ABSTRACT PUBMED 15941357

About this Structure

2AR9 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Engineering a dimeric caspase-9: a re-evaluation of the induced proximity model for caspase activation., Chao Y, Shiozaki EN, Srinivasula SM, Rigotti DJ, Fairman R, Shi Y, PLoS Biol. 2005 Jun;3(6):e183. Epub 2005 May 10. PMID:15941357

Page seeded by OCA on Tue Jul 29 16:22:23 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ar9"
Personal tools