2au7

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[[Image:2au7.gif|left|200px]]
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{{STRUCTURE_2au7| PDB=2au7 | SCENE= }}
{{STRUCTURE_2au7| PDB=2au7 | SCENE= }}
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'''The R43Q active site variant of E.coli inorganic pyrophosphatase'''
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===The R43Q active site variant of E.coli inorganic pyrophosphatase===
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==Overview==
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Here, we describe high-resolution X-ray structures of Escherichia coli inorganic pyrophosphatase (E-PPase) complexed with the substrate, magnesium, or manganese pyrophosphate. The structures correspond to steps in the catalytic synthesis of enzyme-bound pyrophosphate (PP(i)) in the presence of fluoride as an inhibitor of hydrolysis. The catalytic reaction intermediates were trapped applying a new method that we developed for initiating hydrolytic activity in the E-PPase crystal. X-ray structures were obtained for three consecutive states of the enzyme in the course of hydrolysis. Comparative analysis of these structures showed that the Mn2+-supported hydrolysis of the phosphoanhydride bond is followed by a fast release of the leaving phosphate from the P1 site. The electrophilic phosphate P2 is trapped in the "down" conformation. Its movement into the "up" position most likely represents the rate-limiting step of Mn2+-supported hydrolysis. We further determined the crystal structure of the Arg43Gln mutant variant of E-PPase complexed with one phosphate and four Mn ions.
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(as it appears on PubMed at http://www.pubmed.gov), where 17196979 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17196979}}
==About this Structure==
==About this Structure==
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[[Category: Inorganic pyrophosphatase]]
[[Category: Inorganic pyrophosphatase]]
[[Category: Mutant]]
[[Category: Mutant]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:29:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:53:21 2008''

Revision as of 08:53, 29 July 2008

Image:2au7.png

Template:STRUCTURE 2au7

The R43Q active site variant of E.coli inorganic pyrophosphatase

Template:ABSTRACT PUBMED 17196979

About this Structure

2AU7 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Reversible inhibition of Escherichia coli inorganic pyrophosphatase by fluoride: trapped catalytic intermediates in cryo-crystallographic studies., Samygina VR, Moiseev VM, Rodina EV, Vorobyeva NN, Popov AN, Kurilova SA, Nazarova TI, Avaeva SM, Bartunik HD, J Mol Biol. 2007 Mar 2;366(4):1305-17. Epub 2006 Dec 2. PMID:17196979

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