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2aud

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[[Image:2aud.gif|left|200px]]
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{{Seed}}
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[[Image:2aud.png|left|200px]]
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{{STRUCTURE_2aud| PDB=2aud | SCENE= }}
{{STRUCTURE_2aud| PDB=2aud | SCENE= }}
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'''Unliganded HincII'''
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===Unliganded HincII===
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==Overview==
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The 2.1A crystal structure of the unliganded type II restriction endonuclease, HincII, is described. Although the asymmetric unit contains only a single monomer, crystal lattice contacts bring two monomers together to form a dimer very similar to that found in the DNA bound form. Comparison with the published DNA bound structure reveals that the DNA binding pocket is expanded in the unliganded structure. Comparison of the unliganded and DNA liganded structures reveals a simple rotation of subunits by 11 degrees each, or 22 degrees total, to a more closed structure around the bound DNA. Comparison of this conformational change to that observed in the other type II restriction endonucleases where DNA bound and unliganded forms have both been characterized, shows considerable variation in the types of conformational changes that can occur. The conformational changes in three can be described by a simple rotation of subunits, while in two others both rotation and translation of subunits relative to one another occurs. In addition, the endonucleases having subunits that undergo the greatest amount of rotation upon DNA binding are found to be those that distort the bound DNA the least, suggesting that DNA bending may be less facile in dimers possessing greater flexibility.
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The line below this paragraph, {{ABSTRACT_PUBMED_15993893}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15993893 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15993893}}
==About this Structure==
==About this Structure==
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[[Category: Dna binding]]
[[Category: Dna binding]]
[[Category: Restriction endonuclease]]
[[Category: Restriction endonuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:29:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:50:18 2008''

Revision as of 01:50, 29 July 2008

Image:2aud.png

Template:STRUCTURE 2aud

Unliganded HincII

Template:ABSTRACT PUBMED 15993893

About this Structure

2AUD is a Single protein structure of sequence from Haemophilus influenzae. Full crystallographic information is available from OCA.

Reference

DNA-induced conformational changes in type II restriction endonucleases: the structure of unliganded HincII., Little EJ, Horton NC, J Mol Biol. 2005 Aug 5;351(1):76-88. PMID:15993893

Page seeded by OCA on Tue Jul 29 04:50:18 2008

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