2aut

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{{STRUCTURE_2aut| PDB=2aut | SCENE= }}
{{STRUCTURE_2aut| PDB=2aut | SCENE= }}
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'''Crystal structure of Lys154Asn mutant of mature AphA of S. typhimurium'''
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===Crystal structure of Lys154Asn mutant of mature AphA of S. typhimurium===
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==Overview==
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The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA protein was purified to homogeneity. The protein crystallized in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent content of approximately 54%. The crystals are composed of biologically active AphA molecules.
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The line below this paragraph, {{ABSTRACT_PUBMED_17570338}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17570338 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17570338}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
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Structural and mutational analyses reveal the functional role of active-site Lys-154 and Asp-173 of Salmonella typhimurium AphA protein., Makde RD, Gupta GD, Mahajan SK, Kumar V, Arch Biochem Biophys. 2007 Aug 1;464(1):70-9. Epub 2007 May 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17570338 17570338]
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Expression, purification, crystallization and preliminary X-ray diffraction studies of recombinant class B non-specific acid phosphatase of Salmonella typhimurium., Makde RD, Kumar V, Gupta GD, Jasti J, Singh TP, Mahajan SK, Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1849-52. Epub 2003, Sep 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14501135 14501135]
Expression, purification, crystallization and preliminary X-ray diffraction studies of recombinant class B non-specific acid phosphatase of Salmonella typhimurium., Makde RD, Kumar V, Gupta GD, Jasti J, Singh TP, Mahajan SK, Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1849-52. Epub 2003, Sep 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14501135 14501135]
[[Category: Acid phosphatase]]
[[Category: Acid phosphatase]]
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[[Category: Makde, R D.]]
[[Category: Makde, R D.]]
[[Category: Class-b bacterial non-specific acid phosphatase]]
[[Category: Class-b bacterial non-specific acid phosphatase]]
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[[Category: Hydrolase]]
[[Category: Lys154asn mutant of mature apha]]
[[Category: Lys154asn mutant of mature apha]]
[[Category: Metalloenzyme]]
[[Category: Metalloenzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:30:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 27 10:41:44 2008''

Revision as of 07:41, 27 August 2008

Template:STRUCTURE 2aut

Crystal structure of Lys154Asn mutant of mature AphA of S. typhimurium

Template:ABSTRACT PUBMED 17570338

About this Structure

2AUT is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.

Reference

Structural and mutational analyses reveal the functional role of active-site Lys-154 and Asp-173 of Salmonella typhimurium AphA protein., Makde RD, Gupta GD, Mahajan SK, Kumar V, Arch Biochem Biophys. 2007 Aug 1;464(1):70-9. Epub 2007 May 30. PMID:17570338

Expression, purification, crystallization and preliminary X-ray diffraction studies of recombinant class B non-specific acid phosphatase of Salmonella typhimurium., Makde RD, Kumar V, Gupta GD, Jasti J, Singh TP, Mahajan SK, Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1849-52. Epub 2003, Sep 19. PMID:14501135

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