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| - | [[Image:2aw1.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2aw1| PDB=2aw1 | SCENE= }} | | {{STRUCTURE_2aw1| PDB=2aw1 | SCENE= }} |
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| - | '''Carbonic anhydrase inhibitors: Valdecoxib binds to a different active site region of the human isoform II as compared to the structurally related cyclooxygenase II "selective" inhibitor Celecoxib'''
| + | ===Carbonic anhydrase inhibitors: Valdecoxib binds to a different active site region of the human isoform II as compared to the structurally related cyclooxygenase II "selective" inhibitor Celecoxib=== |
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| - | ==Overview==
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| - | The high resolution X-ray crystal structure of the adduct of human carbonic anhydrase (CA, EC 4.2.1.1) isoform II (hCA II) with the clinically used painkiller valdecoxib, acting as a potent CA II and cyclooxygenase-2 (COX-2) inhibitor, is reported. The ionized sulfonamide moiety of valdecoxib is coordinated to the catalytic Zn(II) ion with a tetrahedral geometry. The phenyl-isoxazole moiety of the inhibitor fills the active site channel and interacts with the side chains of Gln92, Val121, Leu198, Thr200, and Pro202. Its 3-phenyl group is located into a hydrophobic pocket, simultaneously establishing van der Waals interactions with the aliphatic side chain of various hydrophobic residues (Val135, Ile91, Val121, Leu198, and Leu141) and a strong offset face-to-face stacking interaction with the aromatic ring of Phe131 (the chi1 angle of which is rotated about 90 degrees with respect to what was observed in the structure of the native enzyme and those of other sulfonamide complexes). Celecoxib, a structurally related COX-2 inhibitor for which the X-ray crystal structure was reported earlier, binds in a completely different manner to hCA II as compared to valdecoxib. Celecoxib completely fills the entire CA II active site, with its trifluoromethyl group in the hydrophobic part of the active site and the p-tolyl moiety in the hydrophilic one, not establishing any interaction with Phe131. In contrast to celecoxib, valdecoxib was rotated about 90 degrees around the chemical bond connecting the benzensulfonamide and the substituted isoxazole ring allowing for these multiple favorable interactions. These different binding modes allow for the further drug design of various CA inhibitors belonging to the benzenesulfonamide class. | + | The line below this paragraph, {{ABSTRACT_PUBMED_16290146}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16290146 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16290146}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Carbonic anhydrase]] | | [[Category: Carbonic anhydrase]] |
| | [[Category: Protein-inhibitor complex]] | | [[Category: Protein-inhibitor complex]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:32:28 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:41:41 2008'' |
Revision as of 10:41, 27 July 2008
Template:STRUCTURE 2aw1
Carbonic anhydrase inhibitors: Valdecoxib binds to a different active site region of the human isoform II as compared to the structurally related cyclooxygenase II "selective" inhibitor Celecoxib
Template:ABSTRACT PUBMED 16290146
About this Structure
2AW1 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Carbonic anhydrase inhibitors: Valdecoxib binds to a different active site region of the human isoform II as compared to the structurally related cyclooxygenase II "selective" inhibitor celecoxib., Di Fiore A, Pedone C, D'Ambrosio K, Scozzafava A, De Simone G, Supuran CT, Bioorg Med Chem Lett. 2006 Jan 15;16(2):437-42. Epub 2005 Nov 14. PMID:16290146
Page seeded by OCA on Sun Jul 27 13:41:41 2008
