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2az3

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{{STRUCTURE_2az3| PDB=2az3 | SCENE= }}
{{STRUCTURE_2az3| PDB=2az3 | SCENE= }}
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'''Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum in complex with CDP'''
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===Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum in complex with CDP===
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==Overview==
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Nucleoside diphosphate kinase from the halophilic archaeon Halobacterium salinarum was crystallized in a free state and a substrate-bound form with CDP. The structures were solved to a resolution of 2.35 and 2.2A, respectively. Crystals with the apo-form were obtained with His6-tagged enzyme, whereas the untagged form was used for co-crystallization with the nucleotide. Crosslinking under different salt and pH conditions revealed a stronger oligomerization tendency for the tagged protein at low and high salt concentrations. The influence of the His6-tag on the halophilic nature of the enzyme is discussed on the basis of the observed structural properties.
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{{ABSTRACT_PUBMED_16293253}}
==About this Structure==
==About this Structure==
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[[Category: Zeth, K.]]
[[Category: Zeth, K.]]
[[Category: Halophilic]]
[[Category: Halophilic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:39:47 2008''

Revision as of 19:39, 27 July 2008

Image:2az3.png

Template:STRUCTURE 2az3

Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum in complex with CDP

Template:ABSTRACT PUBMED 16293253

About this Structure

2AZ3 is a Single protein structure of sequence from Halobacterium salinarum. Full crystallographic information is available from OCA.

Reference

Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum., Besir H, Zeth K, Bracher A, Heider U, Ishibashi M, Tokunaga M, Oesterhelt D, FEBS Lett. 2005 Dec 5;579(29):6595-600. Epub 2005 Nov 9. PMID:16293253

Page seeded by OCA on Sun Jul 27 22:39:47 2008

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