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| - | [[Image:2b0j.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2b0j| PDB=2b0j | SCENE= }} | | {{STRUCTURE_2b0j| PDB=2b0j | SCENE= }} |
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| - | '''The crystal structure of the apoenzyme of the iron-sulfur-cluster-free hydrogenase (Hmd)'''
| + | ===The crystal structure of the apoenzyme of the iron-sulfur-cluster-free hydrogenase (Hmd)=== |
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| - | ==Overview==
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| - | The iron-sulphur cluster-free hydrogenase (Hmd, EC 1.12.98.2) from methanogenic archaea is a novel type of hydrogenase that tightly binds an iron-containing cofactor. The iron is coordinated by two CO molecules, one sulphur and a pyridone derivative, which is linked via a phosphodiester bond to a guanosine base. We report here on the crystal structure of the Hmd apoenzyme from Methanocaldococcus jannaschii at 1.75 A and from Methanopyrus kandleri at 2.4 A resolution. Homodimeric Hmd reveals a unique architecture composed of one central and two identical peripheral globular units. The central unit is composed of the intertwined C-terminal segments of both subunits, forming a novel intersubunit fold. The two peripheral units consist of the N-terminal domain of each subunit. The Rossmann fold-like structure of the N-terminal domain contains a mononucleotide-binding site, which could harbour the GMP moiety of the cofactor. Another binding site for the iron-containing cofactor is most probably Cys176, which is located at the bottom of a deep intersubunit cleft and which has been shown to be essential for enzyme activity. Adjacent to the iron of the cofactor modelled as a ligand to Cys176, an extended U-shaped extra electron density, interpreted as a polyethyleneglycol fragment, suggests a binding site for the substrate methenyltetrahydromethanopterin.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16540118}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16540118 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16540118}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Helix bundle]] | | [[Category: Helix bundle]] |
| | [[Category: Rossmann fold]] | | [[Category: Rossmann fold]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:42:05 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:05:43 2008'' |
Revision as of 18:05, 27 July 2008
Template:STRUCTURE 2b0j
The crystal structure of the apoenzyme of the iron-sulfur-cluster-free hydrogenase (Hmd)
Template:ABSTRACT PUBMED 16540118
About this Structure
2B0J is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.
Reference
The crystal structure of the apoenzyme of the iron-sulphur cluster-free hydrogenase., Pilak O, Mamat B, Vogt S, Hagemeier CH, Thauer RK, Shima S, Vonrhein C, Warkentin E, Ermler U, J Mol Biol. 2006 May 5;358(3):798-809. Epub 2006 Mar 2. PMID:16540118
Page seeded by OCA on Sun Jul 27 21:05:43 2008
Categories: 5,10-methenyltetrahydromethanopterin hydrogenase | Methanocaldococcus jannaschii | Single protein | Ermler, U. | Hagemeier, C H. | Mamat, B. | Pilak, O. | Shima, S. | Thauer, R K. | Vogt, S. | Vonrhein, C. | Warkentin, E. | Helix bundle | Rossmann fold
